QIAprep High-Throughput Plates

QIAprep 96 Turbo Plates and TurboFilter 96 Plates are designed for isolation of up to 20 µg high-purity plasmid or cosmid DNA for use in routine molecular biology applications such as sequencing, cloning, and PCR. The QIAprep 96 Turbo procedure is performed entirely in 96-well format, reducing sample handling to a minimum.

QIAprep 96 Plates together with TurboFilter 96 plates provide automated purification of up to 20 µg molecular biology grade plasmid DNA per well.

QIAprep 96 plates contain a unique silica membrane that binds up to 20 µg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time-consuming phenol–chloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. Pure plasmid DNA eluted from QIAprep 96 plates is ready to use — there is no need to precipitate, concentrate, or desalt.

TurboFilter 96 plates are asymmetric filter units specially designed to allow fast clearing of bacterial lysates generated under high concentrations of chaotropic salts. Crude bacterial lysates are vacuum filtered through the TurboFilter 96 plate, eliminating the need for centrifugation. Particle-free lysates flow directly into the wells of the QIAprep 96 plate, ready for purification and elution. TurboFilter technology streamlines QIAprep plasmid purification and allows the procedure to be fully automated on BioRobot 8000 and Universal System.

Plasmid purification using QIAprep High-Throughput Plates follows a simple bind–wash–elute procedure. First, bacterial cultures are lysed and the lysates are cleared by filtration through TurboFilter plates. The cleared lysate flows directly into the wells of the QIAprep 96 plate, where plasmid DNA adsorbs to the silica membrane. Impurities are washed away and pure DNA is eluted in a small volume of elution buffer or water.

QIAprep 96 Turbo Plates together with TurboFilter 96 Plates provide reproducible yields of pure plasmid DNA suitable for use in most applications, including:

• Sequencing
• Restriction digestion
• Screening
• Ligation
• Transformation