Experiment design
Ensuring quality qPCR
The versatility of quantitative, real-time PCR (qPCR) resulted not only in its ubiquity as a research tool, but also in an abundance of questionable qPCR findings in scientific journals. Without experimental transparency, it’s impossible to assess the integrity of a study or its conclusions. Thus in 2009, the MIQE guidelines were established to address this problem and define the standard for designing and reporting qPCR experiments.
Good qPCR assays display high accuracy, dynamic range, reliability, reproducibility, sensitivity and specificity. In our experience, the primer design algorithm and the reaction buffer conditions must work together to provide the best results. Many researchers design their own assays without full knowledge of the critical design aspects, and often unknowingly compromise their results.
QIAGEN qPCR solutions take the guesswork out of your experiments. Our broad selection of qPCR kits and an unparalleled choice of predesigned assays and panels and the option to design your own using our advanced algorithms ensure quality qPCR results time and again.
QuantiNova kits
Experience the benefits of QuantiNova
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Efficiency gainsSimple and fast procedures that just work right allow you to finish your qPCR jobs sooner and more affordably -
Precise resultsIn-process monitoring tools minimize variation and human error, giving you reliable and reproducible qPCR results -
Flexible optionsChoice of fluorescence detection technology and one- or two-step RT-PCR, compatible with any real-time cycler
Guides and tips for great results
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10 tips to maximize your qPCR successImprove your real-time PCR by simply following these tips and tricks -
Choosing your qPCR detection chemistrySYBR® Green vs. hydrolysis probe: each has its own unique benefits. Which is right for you? -
Biofluid miRNA profiling guidelinesMaximize your miRNA qPCR success for biofluid and liquid biopsy samples -
Avoid common pitfalls in real-time RT-PCRFind out more in our application note: Guidelines for real-time RT-PCR assay optimization
RNA/miRNA profiling
Unparalleled range of assays and panels
Quantitative real-time RT-PCR remains the method of choice for quantification of RNA transcripts, providing flexibility and speed for time-critical assays. Whether you’re assessing miRNA, mRNA or lncRNA expression or verifying the results of NGS studies, our qRT-PCR solutions offer significant benefits for your work.
- High sensitivity: Robust chemistry and an extraordinary offering of assays designed to detect and quantify even the rarest target efficiently.
- High specificity: Proprietary LNA oligo design algorithms and bench verification ensure on-target amplification from a single assay to a full panel.
- Large dynamic range: Whether you have unlimited or precious amounts of total RNA, robust or rare targets, one assay or thousands, our tailored solutions enable detection across the entire spectrum.
- Choice of detection chemistry: Our newest assays and panels are available in both SYBR® Green and hydrolysis probe formats, so you can achieve excellent results no matter which technology you prefer.
Real-time cyclers
Raise your qPCR workflow to the next level
It is increasingly challenging to find the right real-time PCR cycler for your laboratory’s application requirements. For best results, you need low operating costs, high throughput, the ability to multiplex with fast analysis times and reliable reproducibility. As the list of features and specifications grows, an understanding of some of the key performance benefits such as precision, uniformity, dynamic range and resolution becomes critical.
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QIAquant cyclersAll-round systems for high performance in standard or high-throughput real-time PCR applications such as gene expression analysis, genotyping and pathogen detection -
Rotor-Gene QMultiple optimized design features enable outstanding performance and reliable results in specialty applications such as FRET, HRM and 6+plex analysis
Featured resources
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Digital PCRGo beyond the limits of real-time PCR for even higher accuracy, sensitivity and absolute quantification -
Microbiome researchReliable and dedicated solutions that address the specific demands of microbial analysis workflows -
Human ID & forensicsComplete, integrated and optimized workflows for every type of human ID and forensic testing -
HRM applicationsPowerful and cost-effective solutions for analyzing complex sequence variations by high-resolution melting