The Type-it Microsatellite PCR Kit provides fast and reliable genotyping analysis of humans, animals, plants, and bacteria using microsatellites, STR, or VNTR markers, without the need for lengthy optimization procedures. The Type-it Microsatellite PCR Kit is based on proprietary QIAGEN multiplex technology and includes highly specific HotStarTaq Plus DNA Polymerase, a chemically modified hot-start enzyme, and a specially developed multiplex buffer system including Factor MP. Together, these components ensure high yields and enable reliable multiplex amplification of all microsatellite loci of interest. The kit includes dedicated protocols for the amplification of microsatellites or STRs, as well as step-by-step advice on template amounts, cycle numbers, and PCR conditions and instrument details for different downstream analysis platforms such as agarose gels, capillary sequencers, the Agilent Bioanalyzer, and the QIAxcel Advanced System.
Type-it Microsatellite PCR Buffer
Unique Type-it Microsatellite PCR Buffer facilitates the amplification of multiple PCR products. In contrast to conventional PCR reagents, the Type-it Microsatellite PCR Buffer contains a specially developed, balanced combination of salts and additives to ensure comparable efficiencies for annealing and extension of all primers in the reaction. Owing to a uniquely balanced combination of KCl and (NH4)2SO4, the PCR buffer provides stringent primer-annealing conditions over a wider range of temperatures and Mg2+ concentrations than conventional PCR buffers. The need to optimize PCR by varying the annealing temperature or the Mg2+ concentration is dramatically reduced, or often not required. Commonly employed optimization procedures for multiplex PCR are virtually eliminated. The buffer also contains the synthetic Factor MP (see figure " Stable and efficient annealing"), which allows efficient primer annealing and extension, irrespective of primer sequence. Factor MP increases the local concentration of primers at the DNA template and stabilizes specifically bound primers.
The Type-it Microsatellite PCR Kit includes Q-Solution. This innovative PCR additive facilitates amplification of difficult templates by modifying the melting behavior of DNA. Use of this unique reagent will often enable or improve suboptimal PCR. Unlike DMSO and other PCR additives, Q-Solution is used at a defined working concentration with any primer-template system and is not toxic.