QIAamp MinElute Virus Vacuum Kit
Vacuum 과정을 이용하여 serum 과 plasma, cell free body fluid에서 viral DNA와 RNA를 동시에 분리 정제할 수 있습니다
The QIAamp MinElute Virus Vacuum Kit simplifies purification of viral DNA and RNA with fast vacuum procedures. The QIAamp MinElute Virus Vacuum Kit can be used with starting sample volumes up to 0.5 ml. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 μl.
Purified viral nucleic acids are free of proteins, nucleases, and other impurities, and suitable for use in sensitive downstream applications such as PCR and RT-PCR (see figures "High sensitivity in PCR" and "High sensitivity in RT-PCR").
Viral nucleic acids purified using the QIAamp MinElute Virus Vacuum Kit can be used in a wide range of downstream applications, including:
The QIAamp MinElute Virus Vacuum Kit simplifies isolation of viral RNA and DNA from plasma, serum, and cell-free body fluids with a fast vacuum procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp MinElute technology yields viral RNA and DNA from plasma, serum, and cell-free body fluids that are ready to use in PCR and blotting procedures.
The QIAamp MinElute Virus Vacuum Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 µl. QIAamp sample preparation technology is fully licensed.
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp MinElute membrane (see flowchart "Procedure"). Alcohol is added and lysates loaded onto the QIAamp MinElute spin column. Wash buffers are used to remove impurities and viral nucleic acids are eluted in Buffer AVE, ready for use in amplification reactions or storage at –20ºC. Purified nucleic acids are free of proteins, nucleases, and other impurities.
The QIAamp MinElute Virus Vacuum Kit uses well-established technology for simultaneous purification of viral RNA and DNA. Fresh or frozen plasma, serum, and other cell-free body fluids (500 µl) can be processed in less than an hour.
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