FAQ-143073
With which read length should the EpiTect Hi-C NGS libraries be sequenced
Read lengths of 36–150 bp can be used to sequence the EpiTect Hi-C libraries on Illumina sequencers. However, as mappability increases with read length, we suggest the use of 2 x 150 bp sequencing reads for best results.
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EpiTect Hi-C Kit
Hi-C was originally conceived as a powerful technique for genome-wide chromosome conformation capture, enabling the characterization of chromatin folding at kb resolution. However, the technology also has other important applications. For example, Hi-C is used for generating highly contiguous genome assemblies, with few and very long scaffolds, from organisms without a known reference genome. In addition, Hi-C is also very useful for haplotype phasing and detection of chromosomal rearrangements.The EpiTect Hi-C Kit offers a robust, yet simple and fast, protocol with low cell input requirements that enables generation of high-quality Hi-C Illumina NGS libraries from cross-linked cells in less than 2 days.