QIAseq xHYB Viral and Bacterial Panels

The QIAseq xHYB Respiratory Panel includes probes for whole genome enrichment, detection, and characterization of 89 separate viral targets, including: SARS-CoV-2, influenza viruses, rhinovirus, parainfluenza viruses, enterovirus and more.
The QIAseq xHYB Viral STI Panel offers high quality targeted enrichment, detection and genotyping of HBV, HIV-1, and 19 types of high-risk HPV, including types 16 and 18.
The QIAseq xHYB AMR Panel offers targeted enrichment, sequencing and detection of over 2700 separate AMR gene targets.
The QIAseq xHYB Adventitious Agent Panel offers targeted enrichment for the detection of 132 separate viral targets, including: human adenovirus, norovirus, rotavirus, influenza viruses, SARS-CoV-2, HPV, Epstein-Barr virus, HIV, Zika virus, hepatitis viruses and more.

High-quality, single-box solution
All QIAseq xHYB Viral and Bacterial Panels come with all materials necessary for both cDNA synthesis and target enrichment of all targets. When paired with a QIAseq FX DNA Library UDI Kit you can construct sequencing-ready libraries compatible with Illumina platforms.
Novel probe design
Hybrid capture enriches for the target sequence while allowing tolerance for mismatches that may occur in non-annotated or emerging strains. Hybrid capture allows the efficient targeting of a panel of viral genomes or bacterial genes since the number of probes can be scaled up in a cost-effective manner.
Multiplexing
The QIAseq xHYB Viral and Bacterial Panels enable high-level multiplexing on high-throughput Illumina instruments, such as the NovaSeq, with up to 384 UDIs available.
Data analysis
When paired with QIAGEN CLC Genomics Workbench, the panels deliver data that can be quickly analyzed. Sequence data can be used to identify variants across different samples, as well as to compare with multiple genomes – from consensus reference genomes to one of many genomes that have been uploaded from around the world.

Microbial genomes and genes are highly diverse, and this is especially true of viral genomes. Viral genomes exhibit high diversity in morphology, genome size and genomic organization, as they can be composed of DNA or RNA, and in the case of retroviruses, both. In addition, some viruses can exist as an episome or integrate into the host genome. Genome sizes for human viral pathogens can vary from a few thousand to several hundred thousand bases. Also, they can be organized in a single DNA or RNA molecule or can be segmented into several molecules as found in influenza viruses, which contain 8 single-stranded RNA segments.
In addition to viral genomes, bacterial antimicrobial resistance genes are also highly diverse, reflecting the large number of antibiotic drug classes and mechanisms used for resistance. While deep sequencing is capable of detecting antimicrobial signatures, it lacks the sensitivity of targeted sequencing approaches.
To address the challenges of sequencing microbial genes and genomes, hybrid capture panels have been developed to produce high-quality microbial libraries. The hybrid capture panels are used with libraries that are generated after converting RNA to double-stranded cDNA, and then amplified and indexed using the QIAseq FX DNA Library Kit with unique dual indices (UDIs). Hybrid capture enriches for the target sequence while allowing tolerance for mismatches that may occur in non-annotated or emerging strains. In addition, hybrid capture allows the efficient targeting of a panel of viral genomes or bacterial genes since the number of probes can be scaled up in a cost-effective manner.

QIAseq xHYB Viral and Bacterial Panels are compatible with RNA, DNA and TNA workflows. When starting with DNA, it is possible to start with library construction using the QIAseq FX DNA Library Kit.
Converting RNA into double stranded cDNA
When starting with RNA or TNA, the QIAseq xHYB Viral and Bacterial Panel workflow begins with converting the total RNA in the sample into cDNA. The reverse transcription product is then converted into double-stranded cDNA, which is then used as the input for QIAseq FX DNA Library construction after a QIAseq Bead cleanup.
QIAseq FX DNA Library construction
Purified cDNA product is converted to Illumina-compatible NGS libraries using the QIAseq FX DNA Library Kit . The QIAseq FX DNA Library Kit will also generate libraries from genomic DNA if the sample contains it (either as total nucleic acid or isolated genomic DNA). Purified double-stranded DNA products are enzymatically sheared, the fragmented DNA is end-repaired, and an “A” is added to the 3’-end. The product is ready for adapter ligation where Illumina-platform–specific adapters are ligated to both ends of the DNA fragments. The adapters contain the necessary sequences to allow libraries to bind to the flow-cell for sequencing. Following adapter ligation, the libraries are purified using QIAseq Beads, which remove any free adapters. The libraries are then amplified to generate sufficient yields that will go into the hybrid capture reaction.
Hybrid capture
Once the pre-capture libraries are generated, the libraries are hybridized to the QIAseq xHYB probes. After an overnight hybridization, probe-target hybrids are then bound to streptavidin-coated magnetic beads and washed to remove any unbound library fragment. Enriched libraries are then amplified and prepared for sequencing on Illumina systems.
Analysis
Resulting FASTQ can be analyzed via the analysis portal on GeneGlobe or via the QIAGEN CLC Genomics Workbench. Analysis via GeneGlobe is included for free with QIAseq xHYB Viral and Bacterial Panels. It offers a simplified overview of the sequencing results for identification and quantification of either viral species or antimicrobial resistance genes detected in any samples. CLC Genomic Workbench is also available for a deeper analysis for taxonomic identification, variant analysis, phylogenetic trees and viral integration sites.

The QIAseq xHYB Viral and Bacterial Panels allow for the detection, sequencing and characterization of viral and AMR targets. These high-quality libraries will enable:

• Variant detection
• Environmental and population surveillance
• Taxonomic profiling
• Pathogen identification