High recovery of ccfDNA and RNA compared to the QIAamp Circulating Nucleic Acid Kit.
Cell-free DNA/RNA was isolated in duplicates from 1 ml EDTA plasma using the QIAamp ccfDNA/RNA Kit or the QIAamp Circulating Nucleic Acid Kit. Eluate corresponding to 11% of the recovered nucleic acid was used in duplicate 25 µl qPCR reactions to assess DNA recovery. To assess RNA recovery, 17% of each eluate was applied in a total volume of 20 µl for duplicate cDNA synthesis reactions with integrated DNA removal, and 2 µl cDNA were used in a 25 µl qPCR reaction on the Rotor-Gene Q.