Microbial identification and profiling are of interest to many fields including human health, food and feed testing and environmental testing. Microbial identification determines the presence or absence of a microbe in a sample, while microbial profiling determines its relative expression under two or more experimental conditions, thus requiring a reference sample and a normalizer.
Our dPCR Microbial DNA Detection Assays target bacterial, fungal, parasitic, viral, antibiotic resistance or virulence factor genes. For bacteria, the assays target the 16S rRNA gene, and for fungi, they target ribosomal RNA genes. The portfolio consists of over 680 different assays, 200 of which are dPCR wet-lab tested (see assay details in GeneGlobe).
Each assay consists of a primer pair and a hydrolysis probe with a configurable fluorophore dye. The available fluorophores are FAM, HEX, ROX, TAMRA and Cy5, and these can be mixed and matched to support analysis of up to five different targets in one multiplex dPCR reaction. We also offer wet-lab tested dPCR 5-plex assay bundles for analyzing common targets of interest; see Applications below for more details. For the dPCR reaction, the assay is combined with the QIAcuity Probe PCR Kit (DNA targets) or the QIAcuity OneStep Advanced Probe Kit (RNA targets).
The kit works in conjunction with the QIAcuity Digital PCR System and the QIAcuity Nanoplates.
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