Get the most out of NGS variant panels – comparing single primer extension (SPE) to a two-primer amplicon approach
NGS panels have the power to give researchers deeper insights into the genes or genomic regions that matter most. However, issues with coverage gaps, primer drop out and errors during amplification can complicate results.
In this webinar, we’ll take a look at how panel design affects specificity, uniformity and coverage. We’ll discuss the difference between a two-primer amplicon and single primer extension approach, and compare how using unique molecular indices (UMIs) to tag original molecules enhances the ability to call low-frequency variants. We’ll also review a head-to-head comparison of both approaches and discuss the application example. Join us for a comprehensive review on how to get the most out of your NGS sequencing panels!