Guidelines for transfection
Off-target effects in RNAi experiments
Optimizing siRNA transfection
Transfection in multiwell plates — preparing a master mix
If you are performing transfection in multiwell plates, prepare a master mix of transfection complexes or of transfection reagent and culture medium (depending on the protocol) for distribution into plate wells.
- Calculate the required volumes of each component and the total volume before you prepare the master mix.
- Prepare 10% more master mix than is required to allow for pipetting errors (i.e., for a 48-well plate, prepare enough master mix for 53 wells).
- Use a repeat pipet to distribute the master mix.