Working with Plasmids

Troubleshooting the plasmid DNA purification

Technical Illustrations

Thi: Hi, I’m having some trouble with the yield that I’m getting after plasmid purification. I’m not sure what I might be doing wrong and was hoping if you could help me troubleshoot?

Dr. Davis: Of course! I’d be happy to help. Please describe the issues that you’re encountering and let’s try to resolve them together.

Numerous factors may contribute to low yields and poor quality of plasmid DNA. To identify the stage at which any issue may have arisen, you can check the steps outlined on Agarose Gel Analysis Of The Purification Procedure.