Working with Plasmids

Essential factors that may affect bacterial culture and growth

Typically, plasmids are extracted from bacterial cultures that have been cultivated with a selective agent, like an antibiotic. The amount and purity of the extracted plasmid DNA can vary based on several elements, such as the plasmid's copy number, the size of the inserted DNA, the bacterial host strain, the volume of the culture, and the type of culture medium used. Learn more from Growth Of Bacterial Cultures page.