QIAGEN UPXome main image
Next-Generation Sequencing

Versatile 3’ RNA-seq and complete transcriptome RNA-seq

Versatile kit for 3’ RNA-seq and complete transcriptome RNA-seq

Gene expression analysis can be challenging due to complex RNA-seq workflows that only offer low-throughput capabilities and demand high sample input amounts. RNA-seq resources and time are often wasted due to rRNA contamination, limiting the ability to capture on-target reads. Navigate these challenges with the help of the QIAseq UPXome RNA Library Kit – a versatile library prep solution for both 3’ RNA-seq and complete transcriptome RNA-seq with low-input and poor-quality RNA samples. Effortlessly increase throughput while reducing waste by >90% with ultraplex pooling of cDNA.

Ultralow input. Ultraplexing. On-target reads.

You no longer need two separate kits to carry out 3’ RNA-seq and complete transcriptome RNA-seq. QIAseq UPXome RNA Library Kits include protocols for library prep for both, starting with total RNA or enriched mRNA. Get the most out of limited RNA amounts and easily increase throughput to maximize efficiency and productivity on Illumina MiSeq, NextSeq and NovaSeq NGS instruments.

No robot? No problem.

Increase efficiency and productivity with ultraplexing, which gives each sample a unique sample barcode and the availability of a lot of sample indices. These features allow you to have a faster workflow and process higher numbers of samples on Illumina NGS instruments. No need for automation to accelerate your workflow and ramp up throughput.

Did you know that ultraplexing lets you reduce disposable plastics by >90%?
One kit, twice the functionality

Expand your pool of applications, customize your workflow and experience ultimate flexibility with this kit. Whether you’re just interested in 3’ RNA-seq or complete transcriptome RNA-seq, achieve high-quality data for applications such as:

  • Gene expression analysis
  • Transcript identification
  • Fusion gene discovery
QIAseq UPXome RNA Library Kits vs. QIAseq UPX 3’ Transcriptome Kits

Wondering how the QIAseq UPXome RNA Library Kits differ from QIAseq UPX 3’ Transcriptome Kits? Take a look at the key differences.

Kit name QIAseq UPXome RNA Library Kits QIAseq UPX 3’ Transcriptome Kits
Type of libraries Both 3’ RNA-seq and complete transcriptome
RNA-seq possible using a single kit
Only intended for 3’ RNA-seq
Input amounts Works with 500 pg to 100 ng of total
RNA or enriched mRNA
Works with single cells,
cell lysate or less than 10 ng total RNA
Total workflow time Efficient, 6-hour library prep workflow Longer workflow
Ribosomal RNA removal Integrated QIAseq FastSelect technology
eliminates mitochondrial and cytoplasmic
ribosomal RNA
Does not include ribosomal RNA removal
Number of samples
that can be ultraplexed
Ultraplex pooling of cDNA means pooling
of 768—18,432 samples per flow-cell lane
and a reduction of waste by >90%
UPX tagging allows 4608—18,432 samples
per single sequencing lane
generic webinar image, male person sitting in an office watching a webinar, labtop, screen, work on comuter
Watch the webinar

Title: How to resolve challenges with low-input RNA-seq library prep – maximizing whole transcriptome sequencing and analysis using a flexible and customizable strategy
Speaker: Samuel Rulli, Ph.D., Director, Global Product Management, RNA-seq profiling, NGS assay technologies, QIAGEN

Uncovering the connection between arsenic and type II diabetes
"QIAGEN's UPX 3’ Transcriptome service allowed us to generate a large-scale RNA-seq data set that facilitated our discovery of key gene expression changes that drive our disease model."

Have a look at the exciting new research by Dr. Matthew Dodson and Dr. Donna Zhang from the University of Arizona, who investigated the role of Nrf2 regulation in diabetic disease.

[Webinar] Uncovering the connection between arsenic and type II diabetes
Matthew Dodson, Ph.D., Post-Doctoral Researcher, University of Arizona