Fabienne Desmots-Loyer
Next-Generation Sequencing

The importance of rRNA removal in RNA-seq optimization

QIAseq FastSelect: Increase your unique reads, discover more biology

Did you know that a critical step for RNA-seq optimization is efficient removal of rRNA? The amount of rRNA removed is directly proportional to the number of unique gene reads obtained from your library. Efficient, near complete rRNA removal prior to sample cDNA synthesis ensures you aren't making unwanted ribosomal cDNA. Introduce our game-changing QIAseq FastSelect technology into your RNA-seq workflow and experience highly efficient rRNA removal in as little as 14 minutes. In fact, samples treated with QIAseq FastSelect don't require pre-sequencing PCR amplification – simply use your library as is, save even more time and obtain a higher number of unique gene reads. FastSelect your sample before using your library preparation kit and discover more biology.

Discover the missing ingredient in your RNA-seq workflow

Top 5 reasons why QIAseq FastSelect should be part of your RNA-seq workflow
  1. It’s super fast (rRNA removal in 14 min or <1 hour, depending on organism)
  2. It removes >95% rRNA/globin mRNA
  3. It improves RNA-seq sensitivity
  4. It enables deeper gene expression insights
  5. It can be used with stranded RNA-seq library kits from multiple vendors
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Inspiration from the lab bench
QIAseq FastSelect enables high-quality RNA-seq from mixed human and bacterial samples

Dr. Marie Adams, Genomics Core Director at Van Andel Institute, discusses how QIAseq FastSelect technology helped conquer the challenges of generating high-quality RNA-seq data for human and bacterial co-expression studies from the same RNA sample.

Houston lab, people
University of Huston's Department of Biology and Biochemistry
QIAseq FastSelect helps pave the way towards more successful cancer treatment
“QIAseq FastSelect has been phenomenal with the RNA sequencing in my project. The RNA was degraded and almost unusable, but QIAseq FastSelect really removes the ribosomal RNA in these degraded samples and has improved our sequencing libraries”.

Brandon Mistrella, University of Houston's Department of Biology and Biochemistry

Cancer researchers at the University of Houston routinely encountered challenges associated with RNA degradation when working with FFPE samples. The presence of unwanted RNA species such as ribosomal RNA and globin mRNA in the low-yield RNA sample further complicated RNA sequencing. Read how QIAseq FastSelect transformed their research, allowing them to retrieve useful RNA-seq data from FFPE RNA.

Dr. Fabienne Desmots-Loyer, Hematology researcher, CHU – Hospital Pontchaillou, Rennes, France
QIAseq FastSelect enables construction of RNA-seq libraries from low-quality FFPE RNA
“The workflow is very simple and fast. I would say that with poor-quality samples and with the limited quantity of those samples, I wasn’t expecting the results we obtained.”

Dr. Fabienne Desmots-Loyer, Hematology researcher, CHU – Hospital Pontchaillou, Rennes, France

Due to high levels of degradation and poor yields, working with RNA from FFPE samples can be tough. Read the story of Dr. Fabienne Desmots-Loyer at the Hospital Rennes and find out how QIAseq FastSelect saved the day by providing high-quality libraries from FFPE cancer samples previously rejected for processing by two commercial labs due to high levels of RNA degradation and low yields.

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One small step in RNA-seq, one giant leap for your research

QIAseq FastSelect
Dramatically faster and easier rRNA removal

While alternative rRNA removal methods take several hours, require multiple steps, work solely on unfragmented RNA or simply don’t remove enough rRNA, with the FastSelect method you benefit from a dramatically faster and more convenient workflow – and exceptional results even with FFPE and fragmented RNA samples.

Unravel host-microbiome interactions to advance coronavirus metatranscriptomics research

Working with mixed human and microbiome samples from nasopharyngeal, lung and other tissues for coronavirus metatranscriptomics studies? To maximize unique mRNA/gene expression reads, use QIAseq FastSelect –rRNA HMR Kits and QIAseq FastSelect –5S/16S/23S Kits separately or in combination for fast, effective and targeted removal of both host and bacterial rRNA – while still retaining viral RNAs.

Recent SARS-CoV-2 publications using QIAseq FastSelect:

All photos taken prior to COVID-19