Nuclease-Free Water

For use in any molecular biology application


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Nuclease-Free Water (5 liters)

Cat. No. / ID:  129117

5 liters nuclease-free water prepared without the use of diethylpyrocarbonate (DEPC); Provided in five 1 liter bottles, delivered in a cardboard box.
5 liters
10 x 50 ml
1000 ml
The Nuclease-Free Water is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

Nuclease-Free Water is pure, quality-tested water suitable for use in all experiments that require nuclease-free water, including molecular biology applications. Nuclease-Free Water is prepared in a proprietary process, which yields DNase, RNase, and nuclease-free, deionized water without the use of chemical additives, such as diethylpyrocarbonate (DEPC). Nuclease-Free Water is provided in nuclease-free containers.

Nuclease-Free Water is no longer available in a 5 liter bag with luer cap (cat. no. 129117). The required amount is supplied in five 1 liter bottles.


Safety Data Sheets (1)
Kit Handbooks (2)
Highly pure, nuclease-free water for use in all molecular biology applications
Certificates of Analysis (1)


What quality level does your Nuclease-Free Water have?

Nuclease-Free Water has been prepared without the use of chemicals such as DEPC (diethylpyrocarbonate) using an in-house method. The high quality of the water is assured by testing for DNase, RNase, and microbial contamination during the production process.

FAQ ID -1292
Is your Nuclease-Free Water fluorescence-free?

Yes, QIAGEN's Nuclease-Free Water is distilled water that is completely free of substances that may fluoresce.


FAQ ID -1291
What buffer should be used to dilute cDNA made using the miScript Reverse Transcription Kit?

cDNA generated with the miScript Reverse Transcription Kit can be diluted either with Nuclease-Free Water or TE buffer.



FAQ ID -1601
What is the pH value of QIAGEN's Nuclease-Free Water?

At 22°C, Nuclease-Free Water has a pH value of between 5.0 and 6.5. It is not possible to determine the pH of highly pure water exactly. Therefore, many publications/industry standards do not provide a pH specification for highly pure water. Highly pure water does not contain enough ions or impurities for an exact pH determination. In general, values between pH 5 and 8 are obtained.

FAQ ID -1290