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Cloning and Recombinant DNA Technology

Enzymes used for cloning and recombinant DNA technology

Enzymes are essential in cloning and recombinant DNA technology, empowering researchers to manipulate DNA sequences and create recombinant DNA molecules for diverse applications. Restriction enzymes recognize specific DNA sequences and cut the DNA at or near these sites, generating DNA fragments with sticky and blunt-ends that can be ligated into a plasmid vector. DNA ligase joins both sticky and blunt-end fragments. Polymerases amplify DNA fragments by PCR, and Taq polymerase is commonly used for its stability at high temperatures. Reverse transcriptase generates cDNA from RNA templates. 

These enzymes play a vital role in gene cloning, protein expression, gene editing and many other areas of molecular biology research. Browse the entire portfolio and choose the best enzyme that suits your experiment.

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