Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits?
FAQ ID -1553

Yes. QuantiTect Primer Assays are guaranteed for use with QuantiFast SYBR Green PCR and QuantiFast SYBR Green RT-PCR Kits at the specified annealing temperature of 60ºC. We have extensively tested many QuantiTect Primer Assays with success under these conditions.