GeneRead Clonal Amp Q Kit
For clonal amplification of DNA libraries for next-generation sequencing (NGS) applications using the QIAGEN GeneRead QIAcube instrument
Next-generation sequencing (NGS) is a driving force for numerous applications, including cancer research. After the completion of library preparation using the GeneRead DNA Library Q Kit, the GeneRead Clonal Amp Q Kit is used to prepare sequencing templates using clonal amplification.
The GeneRead Clonal Amp Q Kit is for Research Use Only. Not for use in diagnostic procedures.
Please note: The GeneReader NGS System is currently only available with proprietary new sequencing chemistry in the US. Legacy sequencing chemistry is only available ex-US.
The GeneRead Clonal Amp Q Kit enables processing of DNA library samples and pooling of multiple DNA library samples that have been uniquely bar coded during the library preparation process. The clonal amplification workflow is comprised of emulsion making, emulsion PCR, emulsion breaking and the enrichment of live beads. At the completion of the entire clonal amplification workflow, the DNA library sample/pools will have been clonally amplified and, after enrichment, are ready for sequencing.
The 5-step workflow is outlined below:
Library concentration normalization and pooling
Breaking and pooling
Manual determination of bead concentration using OD
The GeneRead Clonal Amp Q Kit is designed for use in the GeneReader NGS System workflow.
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