Ni-NTA Fast Start Kit
For purification and detection of recombinant His-tagged proteins from E. coli lysates
For purification and detection of recombinant His-tagged proteins from E. coli lysates
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Cat. No. / ID: 30600
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
The Ni-NTA Fast Start Kit is based on the remarkable selectivity of patented Ni-NTA (nickel-nitrilotriacetic acid) resin for proteins containing an affinity tag of six or more histidine residues (consecutive or alternating) — the His tag. This technology allows one-step purification of almost any His-tagged protein under native or denaturing conditions. NTA, which has four chelation sites for nickel ions, binds nickel more tightly than metal-chelating purification systems that only have three sites available for interaction with metal ions. The extra chelation site prevents nickel-ion leaching and results in protein preparations with higher purity than those obtained using other metal-chelating purification systems. The His tag also forms the epitope recognized by the Penta·His Antibody supplied with the kit.
The Ni-NTA Fast Start Kit provides reliable, one-step purification of 6xHis-tagged proteins suitable for any application, including:
Features | Specifications |
---|---|
Applications | Proteomics |
Support/matrix | Ni-NTA matrix |
Processing | Manual |
Binding capacity | 5–20 mg/ml |
Special feature | Ni-NTA technology |
Gravity flow or spin column | Gravity flow |
Start material | Cell lysate |
Tag | 6xHis tag |
Yield | <10 mg protein per column |