Cat. No. / ID: 339517
Antisense LNA GapmeRs are highly potent, single-stranded antisense oligonucleotides (ASO) for silencing of lncRNA and mRNA in cell cultures and even in animal models. Antisense LNA GapmeR Positive Controls enable optimization of conditions for lipid-based transfection, electroporation or unassisted delivery. The GapmeRs are enhanced with LNA technology and are designed using sophisticated and empirically developed algorithms and offer excellent performance and high success rates.
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Antisense LNA GapmeRs are powerful tools for protein, mRNA and lncRNA loss-of-function studies. These single-stranded, antisense oligonucleotides (ASOs) catalyze RNase H-dependent degradation of complementary RNA targets. The Antisense LNA GapmeRs are 16 nucleotides long and are enriched with LNA in the flanking regions and DNA in an LNA-free central gap, hence the name "GapmeR" (see figure A unique short, single-stranded antisense design). The LNA-containing flanking regions confer nuclease resistance to the antisense oligo, while also increasing target binding affinity, regardless of the GC content. The central DNA "gap" activates RNase H cleavage of the target RNA upon binding. Antisense LNA GapmeRs have fully modified phosphorothioate (PS) backbones, which ensure exceptional resistance to enzymatic degradation.
Antisense LNA GapmeRs are designed using our empirically derived design tool that incorporates more than 20 years of experience with LNA design. For each RNA target, the tool evaluates thousands of possible designs against more than 30 design parameters to identify the Antisense LNA GapmeRs most likely to provide potent and specific target knockdown.
The primary design parameters include the following:
Antisense LNA GapmeRs can be designed for any RNA target >80 nucleotides and are available in several different grades, depending on your application:
We also offer higher-purity Antisense LNA GapmeRs for use in in vivo studies. These Antisense LNA GapmeRs have excellent pharmacokinetic and pharmacodynamic properties and offer effective and long-lasting RNA silencing in a broad range of tissues. They are well-tolerated and show low toxicity in vivo, due to the following unique features:
|Stage||Design||Screening||in vitro Optimization||in vivo Studies|
|Solution||Online GapmeR design tool||Antisense LNA GapmeR Standard or GapmeR Custom Plates||Antisense LNA GapmeR Premium||Antisense LNA GapmeR in vivo Ready||Antisense LNA GapmeR in vivo Large Scale|
3' and 5' FAM
|Antisense LNA GapmeR Standard||339511
|No purity guarantee||5 nmol
|Antisense LNA GapmeRPremium||339517
|Antisense LNA GapmeR in vivo Ready||339523
|Antisense LNA GapmeR in vivo Large Scale||339532||Custom||Custom, from
5 mg to 1 kg
|Antisense LNA GapmeR Custom Plates||339530||No purity guarantee||0.2 nmol||yes||yes||no||no||no|
These are the available controls.
FAQ ID - 3739