EZ2 AdnaTest CTC Select Kit

For automated immunomagnetic enrichment and integrated mRNA isolation for molecular characterization of circulating tumor cells (CTCs) from whole human blood

Features

  • Automated enrichment of CTCs from whole blood on EZ2 Connect
  • Integrated mRNA isolation for molecular characterization of CTCs
  • High specificity and sensitivity
  • Minimize leukocyte bias with AdnaWash Leukocyte Reducer
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EZ2 AdnaTest CTC Select Kit (12)

Cat. No. / ID: 395692

For 12 preps: For immunomagnetic enrichment of circulating tumor cells (CTCs) from human whole blood
A$3,081.00
KitAccessoriesPrimerMix
EZ2 AdnaTest CTC Select
Filter-Tips and Holders
Accessories
EMT-2 Add On
The EZ2 AdnaTest CTC Select Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

The EZ2 AdnaTest CTC Select Kit provides immunomagnetic enrichment of circulating tumor cells from human whole blood on the EZ2 Connect instrument. Integrated mRNA isolation allows molecular characterization of CTCs using RT-PCR, real-time RT-PCR, or cDNA generation, including the AdnaTest procedure for molecular characterization of CTCs.

Performance

EZ2 AdnaTest CTC Select Kit allows the automated enrichment of CTCs from human whole blood based on the AdnaTest principle using the EZ2 Connect instrument. The EZ2 AdnaTest CTC Select Kit is a highly specific immunomagnetic cell-selection system for enriching circulating tumor cells (see Table "Performance comparable to manual  protocol – EZ2 AdnaTest CTC Select specificity and recovery") from peripheral blood including integrated mRNA isolation. The EZ2 Connect automates the CTC enrichment and subsequent mRNA isolation using prefilled cartridges. The benchtop instrument automatically pierces the sealed cartridges, and automates pipetting of beads and buffers. Its intuitive touchscreen interface makes it easy to use, even for new lab workers. And with its remote connectivity feature, users can monitor runs and the instrument can be managed even from outside the lab (see figure " Easy to use – enrich circulating tumor cells for molecular analyses”).

 

Performance comparable to manual protocol – EZ2 AdnaTest CTC Select specificity and recovery
Method Manual Automated
  AdnaTest CTC Select and BreastCancer Add-on  EZ2 AdnaTest CTC Select and BreastCancer Add-on 
Genes Her2 (265 bp)
Muc1 (299 bp)
Ga733-2 (395 bp)
Her2 (265 bp)
Muc1 (299 bp)
Ga733-2 (395 bp)
Non-spiked healthy donor samples 0/6 0/6
Specificity 100% 100%
Spiked healthy donor samples 6/6 6/6
Recovery 100% 100%
See figures

Principle

EZ2 AdnaTest CTC Select provides prefilled cartridges containing all buffers for the enrichment and lysis of CTCs. A fresh blood sample is incubated with CTC Select beads before loading the sample into the EZ2 Connect instrument. The instrument automatically pierces the prefilled cartridges and enriches the tumor cells using in-tip magnetic bead separation. AdnaTest CTC Select enables the immunomagnetic enrichment of tumor cells from whole blood via epithelial- and tumor-associated antigens. Antibodies against epithelial- and tumor-associated antigens are conjugated to magnetic beads for labeling of tumor cells. Labeled cells are extracted by the EZ2 Connect and lysed. The cartridges also contain oligo (dT) 25-coated beads for isolation of mRNA from the lysate of pre-enriched tumor cells (see figure " During the run"). Reverse transcription results in cDNA that can be used as template for tumor cell detection and characterization by PCR.

Importantly, the AdnaTest is an open platform that allows the profiling of additional custom-selected mRNA targets for comprehensive biomarker discovery.

See figures

Procedure

Fresh blood samples are incubated with CTC Select beads before being loaded into the EZ2 Connect instrument. Oligo dT beads and an elution tube are added to the prefilled EZ2 AdnaTest CTC Select cartridges before loading together with the preincubated samples into the EZ2 Connect instrument. Instrument setup is finalized by adding the large volume rack (cat. no. 9027011) and adding tips and tip holders (provided separately, cat. no. 994900). The EZ2 AdnaTest CTC Select protocol is selected and the instrument started (see figure:  Getting started).

See figures

Applications

The high-quality mRNA purifed can be used in RT-PCR, real-time RT-PCR, cDNA generation, or the AdnaTest procedure for molecular characterization of CTCs. It is suitable for a wide range of applications, including:

  • Isolation of CTCs from whole human blood
  • Molecular characterization of CTCs
  • Biomarker discovery
  • mRNA profiling
  • Liquid biopsy
  • Cancer research

Supporting data and figures

Resources

Kit Handbooks (2)
Brochures & Guides (3)
Step-by-step demos on how to use the EZ2 Connect
Safety Data Sheets (2)
Download Safety Data Sheets for QIAGEN product components.
Download Safety Data Sheets for QIAGEN product components.
Quick-Start Protocols (1)
Operating Software (1)
Version 3

FAQ

What are critical steps during CTC enrichment?

Before use in blood samples, the beads must be pre-washed as described in the manual to remove the sodium azide preservative because it is toxic to cells. Insufficient pre-washing of the beads will likely lead to a loss in sensitivity. 

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How pure are the CTCs enriched from blood samples using the AdnaTest?

After the enrichment process for the CTCs, about 1000–1500 leucocytes remain present (100–150 when using the AdnaTest EMT/SC). However, these low numbers of leukocytes do not interfere with CTC detection in the test procedures since the markers utilized are usually not expressed in leucocytes at detectable levels.

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Can the EZ2 AdnaTest be used for other body fluids than blood?

The EZ2 AdnaTest CTC select is designed for the use with whole blood. Other sample types have not been tested. 

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Can frozen blood be used for the procedure?

The freezing process may compromise the integrity of CTCs; therefore, frozen blood cannot be used.

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Can Heparin blood be used?

Heparin blood cannot be used with the EZ2 AdnaTest CTC Select Protocol since it will lead to clotting of the beads.

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