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MagAttract 96 DNA Plant Core Kit

For efficient, high-throughput, manual or automated purification of total cellular DNA from plant tissue
  • An economical solution
  • Reproducible yields of pure, ready-to-use DNA
  • A simple, rapid, and reliable procedure
  • A manual protocol for initial evaluation

The MagAttract 96 DNA Plant Core Kit combines the efficiency of silica-based DNA purification with the convenient handling offered by magnetic particles, in manual, semi-automated, or automated 96-well plate format.

Cat No./ID: 19090
Buffer RPW (concentrate, 125 ml)
$115.00
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Buffer RPW (concentrate, 125 ml)
Cat No./ID: 67163
MagAttract 96 DNA Plant Core Kit (24)
$7,145.00
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MagAttract Suspension and Buffers for 24 x 96 preps
0
MagAttract 96 DNA Plant Core procedure.
1
Successful PCR analysis.
DNA from different plants was analyzed using the universal trnL PCR System (amplifying an intergenic region of a tRNA coding gene in the chloroplast genome). B: Barley; R: rye; S: sunflower; T: tomato; A: arabidopsis; W: wheat.
2
Reproducible yields.
Maize lysate (30 ml) was generated and divided into aliquots. Each aliquot (containing DNA from 60 mg of wet plant material) was processed using the BioRobot Plant System, and 20 µl of each eluate was loaded onto a 0.8% agarose gel.
3
Reliable real-time PCR analysis.
  
Performance

Purification using the MagAttract 96 DNA Plant Core Kit
Format: 96-well sample plates
Sample sources: Plant tissue
Plant starting material, fresh or frozen: 10–100 mg 
Plant starting material, dried or lyophilized: 10–30 mg
Typical yield: 1–15 µg
Elution volume: 100 µl

Purified DNA has been used successfully in PCR and real-time PCR (see figures "Successful PCR analysis" and "Reliable real-time PCR analysis").

Principle
The MagAttract 96 DNA Plant Kit is designed for fully automated, high-throughput preparation of genomic and chloroplast DNA from plant tissue. Proven MagAttract magnetic particle technology in combination with the BioRobot Plant Science system (no longer available) provides efficient and reproducible DNA purification (see figure "Reproducible yields").
Procedure
Plant samples are mechanically disrupted using a homogenizer such as the TissueLyser. The resulting powder is resuspended in lysis buffer, thoroughly mixed, and centrifuged. Lysates are transferred to a 96-well microplate containing MagAttract magnetic particles, and genomic DNA selectively binds to the surface of the particles (see "MagAttract 96 DNA Plant Core procedure"). After washing, pure genomic DNA is eluted with low-salt buffer, and is ready for use in downstream applications. The MagAttract 96 DNA Plant Kit is designed for use with the BioRobot Plant Science system for fully automated DNA purification. QIAGEN also offers an automated protocol using the BioRobot RapidPlate workstation, and a manual protocol for evaluation purposes.
Applications

The MagAttract 96 DNA Plant Kit provides high yields of DNA from many plant species. The purified DNA is up to 40 kb in size, with fragments of 23 kb predominating, and is ready to use in downstream applications, including:

PCR
Real-time PCR
Microsatellite analysis
SNP genotyping

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Kit Handbooks (1)
(EN) - MagAttract 96 DNA Plant Handbook
For efficient high-throughput isolation of DNA from plant tissue 
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