MagAttract DNA Blood Midi M48 Kit

For automated purification of genomic DNA from blood or buffy coat samples using the BioRobot M48

Features

  • Rapid purification of up to 11 µg high-quality DNA
  • Flexible purification of 6–48 samples per run
  • Easy optimization of DNA concentration

Product Details

The MagAttract DNA Blood Midi M48 Kit provides automated purification of genomic DNA on the BioRobot M48 using proven MagAttract magnetic particle technology. DNA purification and magnetic separation take place in the pipet tips, increasing the efficiency of the procedure. Up to 48 samples can be processed per run in increments of 6 samples. Variable elution volumes enable easy optimization of DNA concentration.

Performance

The MagAttract DNA Blood Midi M48 Kit provides fully automated purification of DNA (see figure " Reproducible purification of high-quality genomic DNA") and the purified DNA is free of PCR inhibitors (see figure  " Reproducible performance in sensitive PCR analysis"). The MagAttract DNA Blood Midi M48 Kit is suitable for use with ACD-, heparin-, and EDTA-preserved whole blood samples (see figure " High-quality genomic DNA from differently preserved whole-blood samples").
See figures

Principle

The MagAttract DNA Blood Midi M48 Kit is designed for fully automated DNA purification. Proven MagAttract magnetic particle technology in combination with the BioRobot M48 workstation provides high-quality DNA, suitable for direct use in sensitive downstream applications. Advantages of the system include scalable sample volumes and elution volumes (see table), giving the yield and concentration of high-quality DNA appropriate for the intended downstream application.

Sample and elution volumes for the MagAttract DNA Blood M48 procedure
SampleKitProtocolAmount of starting material (µl)Elution volume (µl)
BloodMagAttract DNA Blood Mini M48 Kit200 µl Blood100–20050–400
BloodMagAttract DNA Blood Midi M48 Kit350 µl Blood250–350100–400
BloodMagAttract DNA Blood Midi M48 Kit700 μl Blood 500–700200–400
Buffy coat enriched >9xMagAttract DNA Blood Midi M48 Kit75 μl Buffy Coat50–75150–400
Buffy coat enriched ≤9xMagAttract DNA Blood Midi M48 Kit150 μl Buffy Coat100–150150–400
Buffy coat with low leukocyte
concentration
MagAttract DNA Blood Midi M48 Kit300 μl Buffy Coat200–300150–400

Procedure

MagAttract technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. Optimized buffers lyse samples, and DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt (see flowchart " MagAttract DNA Blood Midi M48 procedure"). DNA bound to the magnetic particles is then efficiently washed. Two different wash buffers are used, followed by a rapid rinse with distilled water, which considerably improves the purity of the DNA. High-quality DNA is eluted in the water provided.
See figures

Applications

DNA purified using the MagAttract DNA Blood Midi M48 Kit can be used in a wide range of downstream applications, including:

  • PCR and quantitative, real-time PCR
  • SNP genotyping
  • Sequencing

Supporting data and figures

Specifications

FeaturesSpecifications
applicationsPCR, qPCR, real-time, genotyping, sequencing
formatSample tube (1.5 ml, 2 ml)
forautomatedprocessingBioRobot M48 Workstation
mainsampletypeWhole blood, buffy coat
elutionvolume100–400 µl
processingAutomated
purificationoftotalrnamirnapolyamrnadnaorproteinGenomic DNA
sampleamount250–350 µl
technologyMagnetic particles
timeperrunorperprep28 minutes – 3 hours
yield4.8–11.2 µg

FAQ

How can I prevent running out of M48 Kit reagents before the full number of samples are processed?

We recommend to process a minimum of 24 samples per run on the BioRobot M48 workstation. While the software will allow as few as 6 samples to be run at a time, small amounts of buffer do remain at the bottom of the reagent trays after each run. This may lead to reagent shortage, unless the remaining buffers are being reused.

FAQ ID -1042
Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699
Do you have information on the reproducibility of DNA yields from blood using the M48 BioRobot?
Do we have data on long term stability and storage of DNA purified on BioRobot M48?

DNA isolated from 200 µl blood using the MagAttract DNA Blood protocol and DNA isolated from 10 µl blood using the MagAttract DNA Forensic Trace protocol was shown by PCR after 3 and 4 years, respectively to still be stable.

 

Data is shown here: Storage study for DNA purified on the BioRobot M48

FAQ ID -2611
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12