PAXgene Blood DNA Kit

从全血样本中纯化至多500 μg基因组DNA

S_1393_RPA_PAX1169

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PAXgene Blood DNA Kit (25)

Cat. No. / ID:  761133

Processing tubes and buffers for 25 preparations
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US$456.00
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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 同一体系中进行血液收集和纯化
  • 易于操作
  • 提高工作流程的效率

Product Details

PAXgene Blood DNA系统包括用于血液采集和稳定的PAXgene Blood DNA Tubes和用于单管纯化DNA的PAXgene Blood DNA Kit。

Performance

PAXgene Blood DNA System分离的DNA A260/A280比值在1.7–1.9。片段大小20–200 kb,平均长度50–150 kb(参见" High quality and high molecular weight of genomic DNA")。DNA平均产量为150–500 µg,由血样中的核细胞数目决定。纯化的DNA可用于PCR、定量和real-time PCR(参见" Efficient multiplex PCR of 3 mitochondrial genes")。
See figures

Principle

PAXgene Blood DNA System是整合的标准化体系,用于收集和稳定全血样本并分离基因组DNA。该体系通过PAXgene Blood DNA Tubes收集和稳定血液,并使用PAXgene Blood DNA Kit纯化DNA。

PAXgene Blood DNA Tubes包含可防止血液凝结以及可稳定白细胞的混合试剂,用来收集血液。血液样本可在室温下在管中储存长达14天。用PAXgene Blood DNA Kit中提供的缓冲液获得的高纯度的基因组DNA适合各种下游应用。

Procedure

PAXgene Blood DNA操作步骤如流程图" PAXgene Blood DNA procedure"所示。血液样本(8.5 ml)由PAXgene Blood DNA Tubes收集,并储存或运输。如需纯化DNA,则将血液转入处理管中(已预充细胞裂解缓冲液),将溶液混合,裂解红细胞和白细胞。细胞核和线粒体通过离心收集、洗涤,重悬于裂解缓冲液中。蛋白污染物经蛋白酶孵育后去除。DNA用异丙酮沉淀、70%乙醇洗涤、干燥,并重悬于缓冲液。

See figures

Applications

纯化的DNA可用于多种下游应用,包括:

  • PCR和定量real-time PCR 
  • Southern印迹
  • SNP基因分型
  • 药物基因组研究
  • SNP发现和鉴定

Supporting data and figures

Specifications

FeaturesSpecifications
Main sample typeWhole blood
Time per run60 min/8 samples
Elution volume1 ml
Yield150–500 µg
FormatProcessing tube
Sample amount8.5 ml
Applications(RT-)PCR, (RT-)qPCR, NGS, genotyping, microarray analysis
ProcessingManual (centrifugation)

Resources

产品介绍与指南 (3)
For collection, storage, and transport of whole blood for genomic DNA isolation

 

Second edition — innovative tools
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
试剂盒操作手册 (1)
For isolation of genomic DNA from 8.5 ml human whole blood
Safety Data Sheets (1)
Certificates of Analysis (1)
Brochures & Guides (2)
Second edition — innovative tools
For collection, storage, and transport of whole blood for genomic DNA isolation

 

Gene Expression Analysis (1)
Kit Handbooks (1)
For isolation of genomic DNA from 8.5 ml human whole blood

FAQ

Where can I find additional information for PreAnalytiX PAXgene products?
You can find additional information relating to the PreAnalytiX PAXgene products on the PreAnalytiX website .
FAQ ID - 3515
What does the resuspension buffer, Buffer BG4, in the PAXgene Blood DNA kit contain?

Buffer BG4 consists of 10 mM Tris·Cl, pH 8.5.

FAQ ID - 3501
Does the DNA extracted using the PAXgene Blood DNA System contain RNA?

No. DNA purified with the PAXgene Blood DNA System is free of detectable RNA contamination. In contrast to commercial salting out methods, additional RNase digestion steps are not necessary.

FAQ ID - 3499
How should blood samples drawn into PAXgene Blood DNA Tubes be transported?

See subsequent FAQs for information about storage interval and temperature. Each laboratory should validate shipping procedures.

FAQ ID - 3507
How long can samples in PAXgene Blood DNA Tubes be stored at room temperature?

Samples in PAXgene Blood DNA Tubes can be stored at room temperature (18–25°C) for up to 14 days after blood collection.

FAQ ID - 3508
3497 - What is the expected purity and size of DNA extracted using the PAXgene Blood DNA kit?

The typical A260/A280 ratio is from 1.7 to 1.9. The DNA molecules are up to 200 kb in size, with fragments of 50–150 kb predominating.

FAQ ID - 3497
Can PAXgene Blood DNA Tubes be stored at higher than room temperature (18–25°C)?

Yes, samples in PAXgene Blood DNA Tubes can be stored at 30°C for up to 3 days after blood collection. However, it is not recommended to store blood samples at temperatures higher than 30°C and storage at high temperatures should not exceed 3 days.

FAQ ID - 3509
What is the expected purity of DNA extracted from blood samples collected in PAXgene Blood DNA Tubes IVD?

The typical A260/A280 ratio is from 1.7 to 1.9.

FAQ ID - 3511
Can mitochondrial DNA be isolated with the PAXgene Blood DNA System?

Yes, mitochondrial and genomic DNA are both isolated.

FAQ ID - 3498
Can smaller volumes of blood be processed from the PAXgene Blood DNA Tube?

No. The volume of Buffer BG1 (lysis buffer) prefilled in the processing tubes, and all the other buffer volumes are optimized for use with blood from one PAXgene Blood DNA Tube.

FAQ ID - 3500
Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes IVD?

To date, DNA purified from blood samples in PAXgene Blood DNA Tubes has been successfully tested for use in PCR, including multiplex PCR.

FAQ ID - 3512
How should DNA purified using the PAXgene Blood DNA System be stored?

DNA purified using the PAXgene Blood DNA System should be dissolved in Buffer BG4 and stored at 2–8°C. For long-term storage, DNA can be stored at –20°C but multiple freezing and thawing cycles should be avoided.

FAQ ID - 3502
Is it possible to interrupt the PAXgene Blood DNA kit protocol?

Yes. After dissolving the nuclei pellet in digestion buffer, Buffer BG3 (step 7), samples can be stored up to 7 days at 2–8°C or for one day at room temperature (18–25°C). The protocol can also be interrupted after adding isopropanol (step 10).

FAQ ID - 3503
What downstream applications have been tested with DNA purified using the PAXgene Blood DNA System?

So far, we have shown that DNA purified using the PAXgene Blood DNA System can be used in PCR, including long range and multiplex PCR, restriction digestion and Southern analysis, as well as in various SNP detection procedures including,TaqMan®, LightCycler and Masscode technologies.

See trademarks

FAQ ID - 3506
Can I add bleach to the sample preparation waste?

Do not add bleach or acidic solutions directly to the sample preparation waste. CAUTION: The sample preparation waste contains guanidinium hydrochloride from Buffer BG3, which can form highly reactive compounds when combined with bleach. For more information please consult the appropriate material safety data sheets (MSDS).

FAQ ID - 3505
Can I use the PAXgene Blood DNA Tube to collect blood from animals?

The system is designed for use only with human blood samples.

FAQ ID - 3504
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12