QIAamp Media MDx Kit

用于从液体培养基中自动纯化 DNA

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QIAamp Media MDx Kit (12)

Cat. No. / ID: 965752

用于 12 x 96 次制备：12 QIAamp 96 Plates、缓冲液、Proteinase K、S-Blocks、Disposable Troughs、Racks with Elution Microtubes CL (0.4 ml)、Carrier RNA、Top Elute Fluid、盖、Tape Pad

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QIAamp Media MDx Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

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✓ Knowledgeable and professional Product & Technical Support

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Features

从各种液体运送培养基中纯化
无需离心的全自动操作流程
病毒和其他 DNA 的纯化
高效去除污染物的高品质 DNA

Product Details

QIAamp Media MDx Kit 提供了一种从宫颈拭子运送培养基等液体培养基中纯化 DNA 的全自动操作流程。在 BioRobot MDx 工作站上进行的这一全自动流程包括条形码读取、负载检查和完整的流程记录，96 个样本只需 225 分钟的处理时间，且无需手动操作。

Performance

纯化的核酸不含蛋白质、核酸酶和其他杂质。产量的可重现性高，确保了下游检测结果的准确性（见图“”）。高品质的核酸可随时用于 real-time PCR 等下游检测（见图“”）。

See figures

产量的可重现性高。

高质量核酸的线性产量。

Principle

QIAamp Media MDx Kit 采用成熟的技术从液体培养基中纯化核酸。该试剂盒将硅胶膜的选择性结合特性与高通量 96 孔格式相结合，可在 BioRobot MDx 上全自动同时处理多达 96 个 265 µl 样本。

Procedure

液体运送介质会交联细胞，形成难以裂解的细胞聚集体。QIAamp Media MDx Kit 中经过优化的缓冲液可高效裂解这些样本，稳定核酸，并增强核酸对 QIAamp 膜核酸的选择性吸附（见流程图“”）。加入酒精，并将裂解物加入到 QIAamp 96 孔板。使用洗涤缓冲液去除杂质（包括酒精和磷酸盐），然后在低盐缓冲液中洗脱纯净的即用型 DNA。正在申请专利的计算机控制真空技术可确保高效去除液体细胞学培养基中的挥发性污染物，如酒精。

See figures

QIAamp Media MDx 方案。

Applications

QIAamp Media MDx Kit 可用于从各种来源纯化细胞、细菌和病毒核酸，包括：

含酒精的液体细胞学培养基（如 PreservCyt 和 SurePath）
磷酸盐缓冲液体运送培养基（例如 M4RT）
尿液
干血斑、血液检测试纸和拭子

Supporting data and figures

QIAamp Media MDx 方案。

Specifications

Features	Specifications
Applications	PCR、real-time PCR
Elution volume	120 µl
Main sample type	液体培养基
Processing	自动
Technology	硅胶膜纯化技术
Time per run or per prep	200 分钟（96 个样本）
Sample amount	265 µl
Format	96 孔板
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	病毒 DNA 和 RNA、细菌 DNA 和 RNA、细胞 DNA 和 RNA
For automated processing	BioRobot MDx Workstation
Yield	不一

Resources

For automated purification of nucleic acids from liquid media using the BioRobot MDx workstation

Download Safety Data Sheets for QIAGEN product components.

For automated purification of nucleic acids from liquid media using the BioRobot MDx workstation

FAQ

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

FAQ ID -761

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728