QIAsymphony Virus/Bacteria Kits
从1–96个样本中自动纯化病毒核酸或细菌DNA
Products
QIAsymphony Virus/Bacteria Kits 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。
Features
- 标准化的处理流程保证高度重复性
- 条形码扫描可追踪样本和试剂
- 经优化的操作流程确保高产量高纯度的核酸
- 新型预装试剂条，操作简便
Product Details
QIAsymphony Virus/Bacteria Kit可在QIAsymphony SP核酸提取纯化分析仪上，从多种类型样本中自动纯化病毒核酸和细菌DNA。例如，可以从血浆、血清、脑脊液、呼吸道样本、尿常规样本、泌尿生殖道拭子和运输介质中纯化核酸。试剂盒有小型和中型规格，对应样本体积200 μl和至多1000 μl。经优化的实验方案提供高产量高纯度的核酸，和灵活的洗脱体积，因而可以根据每个下游应用，优化调整核酸浓度。新型的预装试剂条减少了工作站设置时间，并减少了手动操作。条形码阅读器进行试剂全程跟踪。
Performance
QIAsymphony Virus/Bacteria Kit可以准确、重复地的纯化核酸。经纯化的病毒和细菌核酸没有蛋白质、核酸酶和其他杂质，适用于敏感的下游应用。高产量和灵活的洗脱体积（60 μl、85 μl或者110 μl）符合下游应用的要求，适用于敏感的分析检测（参见" Sensitive detection of Chlamydia trachomatis in urine"）。
See figures
Principle
QIAsymphony Virus/Bacteria Kit将硅胶膜纯化病毒和细菌核酸的速度、效率与易于操作的磁性颗粒相结合（参见" Efficient processing of magnetic particles"）。创新的即用型试剂条预装有磁性颗粒和纯化步骤所需的所有试剂，包括附加的酶，以及可在QIAsymphony SP核酸提取纯化分析仪上被自动打开的试剂，可较大程度降低污染的风险（参见" Prefilled, sealed reagent cartridges"）。台面设置快速，节省你的宝贵时间。只需简单地在试剂置入模块中加载至多2个试剂条。试剂条可以来自相同的试剂盒，也可以是不同的试剂盒，因此可在处理96个样本的同一批次中，运行不同的纯化步骤。试剂仅会用于所选数目的样本，实现成本控制。
See figures
Procedure
纯化操作流程的目的在于确保安全、可重复的处理传染性样本，包括4个步骤：裂解、结合、洗涤和洗脱。用户可以选择不同的洗脱体积，取决于实验方案。在QIAsymphony SP核酸提取纯化分析仪上可完成所有纯化流程的步骤。每次运行至多可以处理96个样本。
| 实验方案 | 试剂盒 | 样本材料 | 处理体积 |
|---|---|---|---|
| Cellfree 200 | QIAsymphony Virus/Bacteria Mini Kit (192) | 血浆、血清和脑脊液 | 200 μl |
| Cellfree 500 | QIAsymphony Virus/Bacteria Midi Kit (96) | 血浆、血清和脑脊液 | 500 μl |
| Cellfree 1000 | QIAsymphony Virus/Bacteria Midi Kit (96) | 血浆、血清和脑脊液 | 1000 μl |
| Complex 200 | QIAsymphony Virus/Bacteria Mini Kit (192) | 呼吸道样本（BAL、干拭子、传输介质、呼出物、痰）和泌尿生殖系统的样品（尿液、运输培养基） | 200 μl |
| Complex 400 | QIAsymphony Virus/Bacteria Midi Kit (96) | 呼吸道样本（BAL、干拭子、传输介质、呼出物、痰）和泌尿生殖系统的样品（尿液、运输培养基） | 400 μl |
| Complex 800 | QIAsymphony Virus/Bacteria Midi Kit (96) | 呼吸道样本（BAL、干拭子、传输介质、呼出物、痰）和泌尿生殖系统的样品（尿液、运输培养基） | 800 μl |
Applications
QIAsymphony Virus/Bacteria Kit能够灵敏的检测多种DNA和RNA病毒以及细菌病原体。产量呈线性，准确的定量分析高和低的病毒或者细菌滴度。高品质的核酸可即用于各种下游应用，包括定量real-time PCR或者RT-PCR等敏感的应用。
Supporting data and figures
Sensitive detection of Chlamydia trachomatis in urine.
DNA was purified from 800 μl urine with elution in 110 μl. Bacterial DNA was detected using a commercially available assay amplifying a specific sequence of the C. trachomatis genome on an ABI PRISM 7900HT SDS.
Specifications
| Features | Specifications |
|---|---|
| Applications | PCR, real-time PCR |
| Elution volume | 60-110 µl |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Purification of viral RNA and DNA and bacterial DNA |
| Input volume | 800 µl (Pathogen Complex Protocol); 1000 µl (Virus Cellfree Protocol) |
| Technology | Magnetic particles |
| Sample types | Serum, plasma, CSF, cell-free body fluids, fresh or frozen material (body fluids, swabs, secretions, tissues, stool specimens) |
Resources
安全数据表 (1)
Labware Files and Documents (2)
试剂盒操作手册 (1)
Safety Data Sheets (2)
Certificates of Analysis (1)
Kit Handbooks (1)
FAQ
Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run?
Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?
What sample types can be processed using the QIAsymphony Virus/Bacteria protocols?
What sample types can be processed using the QIAsymphony DSP Virus/Pathogen protocols?
What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits?
Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument?