QIAamp 96 Virus QIAcube HT Kit

用于高通量自动化纯化多种类型样本中的病毒RNA以及DNA

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QIAamp 96 Virus QIAcube HT Kit (5)

Cat. No. / ID: 57731

For 480 preps: QIAamp 96 plates, QIAGEN Proteinase K, carrier RNA, buffers

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QIAamp 96 Virus QIAcube HT Kit

QIAcube HT Plasticware

QIAamp 96 Virus QIAcube HT Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

简单而可靠的自动化处理过程带来了时间和成本的节省
适用于包括血液、组织、拭子以及各种体液在内的多种样本
可确保结果的一致性及高产量
可有效去除样本中的抑制剂及污染物
纯化后的核酸可直接用于实时定量PCR或RT-PCR实验

Product Details

QIAamp 96 Virus QIAcube HT Kit可在QIAcube HT仪器平台上实现简单快速的自动化病毒RNA及DNA纯化。通过使用96孔板形式的高效QIAamp硅胶膜技术进行纯化后，已去除了污染物及抑制剂的高质量核酸可直接用于下游的一系列分析。

Performance

QIAamp 96 Virus QIAcube HT Kit可用于从一系列样本中进行RNA及DNA的自动化纯化，这些样本包括新鲜或冷冻的组织、血液或其他体液样本。纯化所得的高质量病毒核酸可保证在下游的一系列PCR及RT-PCR反应中得到优异的表现。

专为QIAcube HT平台而设计的QIAamp 96 Virus QIAcube HT Kit可帮助实验者在不牺牲任何实验结果质量及可靠性的前提下，提升样本纯化实验的通量。与其他QIAGEN DNA纯化方案类似，该高通量实验步骤可提供高产量的纯化RNA或DNA，从而保证下游分析结果的优异表现。

Principle

QIAamp 96 Virus QIAcube HT Kit将硅胶膜的选择性结合特性与96孔板形式的高通量处理方式进行了有效的结合，专门为QIAcube HT平台上24–96个样本的完全自动化平行处理而设计。

试剂盒参数
参数	描述
样本数	24–96样本（可每8个样本为单位递增）
样本上样体积	血液及其他体液样本：最多200 μl（对于体积小于200 μl的样本,可通过添加PBS补齐）组织：最多20 mg组织样本
洗脱体积	100 μl
处理时间	96样本约需145分钟 24样本约需90分钟

Procedure

在QIAcube HT仪器的帮助下，QIAamp 96 Virus QIAcube HT试剂盒操作步骤简单而快速。样本在QIAGEN蛋白酶K及Buffer ACL形成的高度变形条件作用下可实现常温下的有效裂解，并同时确保核酸酶的活性抑制。Buffer ACB的加入还可用于调节DNA与RNA同时纯化的结合条件。之后，裂解液将会被转移到QIAamp 96孔板中，在真空压力的作用下，溶液中的核酸将会被吸附到硅胶膜上而其他污染物则会流过硅胶膜。随后三步有效的洗涤步骤将确保剩余污染物以及酶抑制剂的去除，最后核酸将会被洗脱到Buffer AVE中。某些特殊样本可能需要样本前处理过程。

Applications

纯化所得的高质量核酸可直接用于所有的下游检测应用中，包括利用实时定量PCR或RT-PCR的高灵敏度检测反应。

Supporting data and figures

Reliable automated purification.

Viral nucleic acids were purified from 200 µl plasma samples spiked with 10,000, 1000 and 100 IU/ml of a typical DNA or RNA virus. Sample processing was automated using either QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol, QIAxtractor with the VX Protocol, or QIAcube with the QIAamp MinElute Virus Spin Kit. Viral nucleic acids were detected using in-house PCR and RT-PCR assays, with 20 µl eluate per reaction on the Rotor-Gene Q. Results show that QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit performs as well as or better than the other methods.

High sensitivity of purified viral nucleic acids in PCR and RT-PCR.

Reliable purification from a range of sample types.

Resources

For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17

For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software

Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.

Q Protocol notes for purification of viral nucleic acids from human samples on the QIAcube HT using the QIAamp 96 Virus QIAcube HT Kit; use with the QIAamp 96 Virus QIAcube HT V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.

Download Safety Data Sheets for QIAGEN product components.

For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17

For use with QIAcube HT Operating Software 4.17; not compatible with QIAcube HT Prep Manager Software

For use with the QIAcube HT Prep Manager Software

For QIAcube HT and QIAxtractor

For fast and reliable automated 96-well nucleic acid purification

For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17

For use with QIAcube HT Operating Software 4.17; not compatible with QIAcube HT Prep Manager Software

For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17

For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software

Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.

For QIAcube HT and QIAxtractor

For use with the QIAcube HT Prep Manager Software

For QIAcube HT and QIAxtractor

FAQ

No, the two software applications are not interchangeable.

Additionally, the QIAcube HT and QIAxtractor software cannot be installed on the same computer. The QIAcube HT software versions is v4.17 or higher, while the QIAxtractor software version is v4.16 or lower.

FAQ ID -9102

The QIAcube HT Plasticware content is calculated for use with the extraction protocol working in batches of 96 samples. If running smaller batches and/ or the transfer protocol, additional consumables are required.

FAQ ID - 4010

No, this upgrade is not possible.

FAQ ID -9104

The Support Packages are important when reporting an issue to QIAGEN Technical Services. Upon completion of each run, the software automatically saves a support package.

To retrieve a support package, click Help in the QIAcube HT or QIAxtractor software and select Choose support package. In the new windows, select the support package that corresponds to the issue and send it to QIAGEN Technical Services.

FAQ ID -9100

1. Check the vacuum tubing to make sure it is not pinched or obstructed in some way. Sometimes the tubing can become pinched against the bench top when the vacuum station is placed too far from the instrument.

2. Make sure that the channeling adapter is properly placed. Check if the carriage is moving the plate completely from the waste chamber position to the elution chamber position. If the calibration seems incorrect, the instrument may need to be re-taught at this position.

3. When using less than a full plate, make sure all the unused columns are covered with an adhesive seal.

Also, make sure each column of 8 samples is full. When processing a number of samples that results in < 8 samples per column, use water as a substitute in the empty positions until the column is filled. This ensures even vacuum across all unsealed well positions.

4. Make sure correct plates are used for the selected protocol.

5. When the vacuum is on, please listen for any hissing noise from the tubing that is coming down to the waste container. This could indicate leaks in the tubing.

If the issue persists, please contact QIAGEN Technical Service.

FAQ ID -9106

Make sure the QIAcube HT or QIAxtractor software uses the same COM port number as assigned to the QIAcube HT or QIAxtractor instrument on the computer. The COM port setting can be checked by clicking Options\Robot Setup\Select COM port in the QIAcube HT or QIAxtractor software.

Ensure that the cable between the instrument and the computer is securely inserted. Power on the instrument first, and then launch the QIAxtractor/QIAcube HT software.

The instrument has both RS-232 (serial) and USB interfaces for computer connection. Try an RS-232 cable if the communication cannot be established between the instrument and computer with a USB cable.

If the issue persists, please contact QIAGEN Technical Services.

FAQ ID -9101