MaXtract High Density

用于从有机溶剂更安全、方便地提取核酸

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MaXtract High Density (200 x 2 ml)

Cat. No. / ID: 129056

200 x 2 ml MaXtract High Density 管

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Quantity

200 x 2 ml

200 x 1.5 ml

100 x 15 ml

25 x 50 ml

MaXtract High Density 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

从有机溶剂中更安全地提取核酸
减少携带污染物
高核酸回收率
核酸回收方便

Product Details

MaXtract High Density 可简化从有机溶剂（例如，苯酚/氯仿）中提取核酸的过程。MaXtract 凝胶可在有机溶剂与含核酸的水相之间形成稳定的屏障，从而方便地回收水相，同时防止携带有机溶剂、蛋白质及其他污染物。

Performance

与传统提取方法相比，MaXtract High Density 能够将回收率提高 30%。蛋白质及其他污染物迁移至有机相和相间。MaXtract High Density 凝胶形成的屏障足够耐用，以致于这些污染物仍被截留在屏障下方，从而防止在去除水相时携带污染物。水相可通过倒出或移液去除，从而使得核酸回收率与使用传统有机提取方法时相比高 30%。

MaXtract High Density 有多种管尺寸提供，支持从 100 µl 至 20 ml 样本体积中提取。

MaXtract High Density 相分离
水相	苯酚/氯仿	氯仿
＜0.5 M NaCl，＜1 mg/ml 蛋白质	是	是
≥0.5 M NaCl	是	是
≥1 mg/ml 蛋白质	是	是
质粒 DNA 分离	是	是
基因组 DNA 分离	是	是
RNA 分离	是	是

* 包括苯酚/氯仿/异戊醇。

Principle

MaXtract High Density 凝胶使用苯酚/氯仿等溶剂，从有机提取物中安全、快速回收核酸（参见流程图“”）。

See figures

MaXtract 程序。

Procedure

仅需将核酸溶液和有机溶剂添加到含 MaXtract 凝胶的试管中。混合和离心之后，MaXtract High Density 凝胶会形成稳定的屏障，从而将有机相和水相分开。屏障可安全捕获有害有机溶剂和污染物，让您轻松地将含核酸水相倒出或移液至新试管中（参见图“ 安全、轻松的核酸提取”）。如需要第二次提取，则可在同一试管中进行，前提是未超过最大试管容积。

使用 MaXtract High Density 凝胶进行的分离依赖于水相和有机介质之间的密度差。有机层的密度必须高于凝胶和水相，并且凝胶的密度必须高于水相。

See figures

可实现安全、轻松的核酸提取。

Applications

MaXtract High Density 非常适合用于质粒 DNA、基因组 DNA 或 RNA 分离。还提供经过优化可用于特定应用（如从低熔点琼脂糖中回收 DNA）的方案。使用 MaXtract High Density 凝胶提取的核酸适合用于许多下游应用。

Supporting data and figures

MaXtract 程序。

MaXtract 程序可靠、便捷。

Resources

Download Safety Data Sheets for QIAGEN product components.

For improved recovery of nucleic acids during organic extraction procedures

FAQ

Yes. The organic phase can be placed in MaXtract Low and High Density tubes without any problem 3-4 hours before preparation of the samples. Make sure to perform the centrifugation step first, so that the gel collects at the bottom of the tube.

FAQ ID -1299

No. After autoclaving, the MaXtract gel no longer has the same properties. Since the MaXtract tubes are manufactured and filled in a fully automated process, autoclaving is not required.

FAQ ID -1305

DNA purified by organic extraction can principally be used for these downstream reactions. It should be noted that phenol, like all organic solvents, alters the active centre of enzymes, thus deactivating them. An ethanol precipitation should therefore generally be carried out prior to enzyme controlled downstream reactions. Potential phenol residue is thereby effectively removed.

The use of MaXtract Low and High Density tubes has the advantage that no recontamination of the sample by organic solvents or proteins occurs.

FAQ ID -1303

Generally, this is determined by the density of the denatured proteins and the gel type in the MaXtract Tubes. If proteins show a higher density than the gel, they will accumulate underneath in the organic phase. If they have the same density as the MaXtract gel, they will collect in the gel. The quantity and ratio of phenol:chloroform can also influence the behavior of the proteins.

FAQ ID -1312

Yes, we have the following protocols:

Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueRuptor (RY29).
Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueLyser (RY30).
Purification of total RNA from fatty tissues using the RNeasy Lipid Tissue Mini Kit and MaXtract High Density (RY31).

FAQ ID -1550

Yes, this application is possible with both MaXtract Low and High Density Tubes. A sufficient quantity of BCP should be used when using MaXtract High Density, in order to achieve a stable gel phase. BCP increases the density of the organic phase in the same way as chloroform.

-3

No. The functional efficiency of the MaXtract Low and High Density Tubes does not depend on the pipetting sequence.

FAQ ID -1307

If organic solvents are added immediately following the filling of MaXtract Low and High Density Tubes with the frozen sample, it should not have any negative effect on the functionality of the tubes.

FAQ ID -1301

Yes. When using MaXtract Tubes with QIAzol Lysis Reagent, please follow the User-Developed protocol 'Purification of total RNA from fatty tissues using the RNeasy Lipid Tissue Mini Kit and MaXtract High Density' (RY31) .

FAQ ID -1304

MaXtract High Density gel is found in the yellow tubes, and MaXtract Low Density gel is contained in the green tubes.

FAQ ID -1315

Yes, if a sufficiently large MaXtract tube has been selected for the extraction it is possible to perform multiple purification steps in this tube. Sample and organic solvent are placed in the MaXtract tube, mixed and centrifuged. Organic solvent is then added again to the aqueous phase separated by the gel, followed by mixing and centrifuging. Following the last extraction step, the sample is transferred to a new tube for storage.

For the purification of samples with high protein content, we recommend using a new MaXtract tube for each extraction step.

FAQ ID -1302

CTAB as a rule increases the density of the aqueous phase. Therefore, MaXtract High Density should be used for the organic extraction of DNA from CTAB samples.

Depending on the amount of CTAB used, the density of the sample can become too high for the MaXtract High Density Tube, leading to a gel barrier above the aqueous phase. In this case, puncture the gel with the tip of a pipette and dilute the aqueous phase with either distilled water or TE buffer. Mix again and then centrifuge. However, we recommend to repeat the separation in a new MaXtract tube.

FAQ ID -1308

A phenol-chloroform ratio of 1:1 is advantageous for the phase separation when using MaXtract Low and High Density, since the MaXtract interphase has the greatest stability at this ratio.

Methods using a phenol-chloroform ratio of as much as 6:1 are also possible with MaXtract. However, MaXtract can partly precipitate onto the bottom of the tube in this case.

If the phenol-chloroform ratio is greater than 1:1, it is necessary to use MaXtract Low Density Tubes. Note that this MaXtract type is not compatible with applications in which the aqueous phase has a high density (e.g., isolation of plasmid DNA and RNA).

FAQ ID -1309

In this case, both MaXtract Low and High Density Tubes can be used with the same success. We principally recommend testing both MaXtract types.

FAQ ID -1310

No, MaXtract Low and High Density tubes are not coated in any way.

FAQ ID -1298