Custom RT2 PreAMP Pathway Primer Mixes
用于Custom RT² Profiler PCR Arrays分析之前扩增cDNA模板
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Custom RT2 PreAMP Pathway Primer Mix
Cat. No. / ID: 330141
Primer mix for a specific RT2 Profiler PCR Array
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Custom RT2 PreAMP Pathway Primer Mixes 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。
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Find or custom design the right target-specific assays and panels to research your biological targets of interest.
Features
- 适用于人类、小鼠及大鼠的定制引物混合液
- 最少的手动操作时间
- 可靠检测少量样本
Product Details
RT2 PreAMP cDNA Synthesis Kit和RT2 PreAMP Pathway Primer Mixes是创新的技术，能够用1 ng的总RNA进行基因表达分析。专有的扩增过程增加了用于PCR芯片分析的cDNA的量。起始样本包括穿刺活检样本、激光捕获显微切割样本、干细胞团或胚胎体及流式细胞分选仪（FACS）分选的细胞。专门设计的引物混合物适用于人类、小鼠或大鼠的Custom RT2 Profiler PCR Arrays。
Performance
阳性信号率更高，无偏差扩增获得无偏向性的结果
更多基因可使用RT2 PreAMP Pathway Primer Mix进行预扩增之后被检测（参见" Increased positive call rate"）。扩增过程无偏向性，回归分析评估发现，预扩增的与未扩增的cDNA之间能产生高度可比的ΔCT值（参见" Unbiased amplification process"）。
在预扩增与未扩增样本之间，基因表达倍数变化有高度相关性（参见" Faithfully amplified biology"）。
See figures
Principle
RT2 PreAMP技术利用多重串联PCR以最小的偏差预扩增基因特异性cDNA。逆转录后，RT2 PreAMP Pathway Primer Mix用于扩增模板，能够在4个不同Custom RT2 Profiler PCR Arrays上进行基因表达分析（参见" Simple template amplification and gene expression analysis"）。
See figures
Procedure
首先使用RT2 PreAMP cDNA Synthesis Kit，同时反转录最多12 种不同的RNA样本成为cDNA。然后将cDNA进行预扩增用于特定的基因列表。每个合成的cDNA第一条链用4种不同的Custom RT2 PreAMP Pathway Primer Mixes进行扩增，能够在4个不同的特定PCR芯片上进行基因表达分析。RT2 PreAMP cDNA Synthesis Kit中含有Side Reaction Reducer，可去除预扩增所产生的引物残留，在RT2 Profiler PCR Arrays进行准确检测。把预扩增的模板与仪器特异性、即用型RT2 SYBR® Green Mastermix混合，完成PCR分析过程。
Applications
使用Custom RT2 PreAMP cDNA Synthesis Kit及RT2 PreAMP Pathway Primer Mixes生成的cDNA模板可即用于Custom RT2 Profiler PCR Arrays进行基因表达谱分析。
Supporting data and figures
Increased positive call rate.
First strand cDNA was synthesized from 40 ng mouse total RNA with (light blue bars) or without (dark blue bars) preamplification with RT² PreAMP PCR Mastermix and RT² PreAMP Pathway Primer Mix. The unamplified and amplified samples were then analyzed on the Mouse Inflammatory Cytokines & Receptors RT² Profiler PCR Array and the threshold CT were obtained. Genes with CT ≥35 were considered to be "absent". In the unamplified sample, 75% of the genes analyzed were present. The positive call rate was increased to 100% in the amplified sample. The 22 genes shown were called "absent" in the unamplified sample and were detectable in the preamplified sample.
Resources
安全数据表 (1)
Safety Data Sheets (1)
Certificates of Analysis (1)