S_1151_8_Strep_tagAntibody

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Strep-tag Antibody (100 μg)

Cat. No. / ID:  34850

可识别 Strep-tag II 表位的冻干小鼠单克隆抗体,用于 1000 ml 工作溶液
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Strep-tag Antibody 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

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✓ Knowledgeable and professional Product & Technical Support

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Features

  • 在点印迹和蛋白印迹程序中效果出色
  • 高灵敏度和特异性检测

Product Details

小鼠 Strep-tag 单克隆抗体用于以高特异性和灵敏度检测携带短 Strep-tag 亲和标记表位的重组蛋白。与所有 QIAGEN 小鼠单克隆抗体一样,它们在无血清条件下制备,因此可确保不含病毒、支原体和污染性免疫球蛋白。

Performance

使用 Strep-tag Antibody 可对蛋白进行高灵敏性检测(见图 以高灵敏性检测携带 Strep-tag 的蛋白 两步纯化超纯蛋白
See figures

Principle

两步亲和纯化系统基于成熟的 6xHis 标签和短 Strep-tag II,能通过快速、标准化的程序简单、高效地纯化超纯蛋白。携带这两种标记的重组蛋白依次在 Ni-NTA 和 Strep-Tactin 基质上进行纯化(见图 两步纯化超纯蛋白)。这两种简单的亲和纯化方法可提供适合任何下游应用的完全活性、全长、超纯蛋白。

See figures

Procedure

带有两个小亲和标记(6xHis 标记和 Strep-tag II)的重组蛋白在大肠杆菌、昆虫或哺乳动物细胞中使用 pQE-TriSystem His·Strep 载体进行高效表达。在细胞裂解和澄清裂解物后,使用基于成熟 6xHis-标签-Ni-NTA 相互作用的固定金属亲和层析程序初步纯化蛋白质。使用咪唑从 Ni-NTA 基质洗脱后,重组蛋白(也携带 Strep-tag II 表位)会被直接加载到 Strep-Tactin 基质上。无需更换缓冲液。使用生物素或去硫生物素从 Strep-Tactin 基质中洗脱蛋白。这种两步亲和纯化法可获得超纯(纯度大于 98% 的)蛋白(见图 两步亲和纯化程序)。纯化顺序可以颠倒(即先纯化 Strep-Tactin,再纯化 Ni-NTA)。使用小鼠单克隆 Strep-tag 或 Anti His 抗体可以高特异性和灵敏度检测蛋白。

See figures

Applications

两步亲和纯化系统使用 Strep-tag Antibody 进行检测,非常适合高纯度成本高昂或难以实现的应用。标准化纯化程序还能提高产量,因为无需再开发和优化针对特定蛋白的纯化方案。两步亲和纯化系统提供了超高纯度和便利性,从而成为以下应用的首选方法:

  • 结构和功能分析
  • 真核系统中的表达

Supporting data and figures

Specifications

FeaturesSpecifications
Applications蛋白印迹、点印迹、ELISA、免疫沉淀、免疫组织化学
Detection需要二抗
Sensitivity in Western blots (chemiluminescent detection)1 ng
Substrate for blot detection取决于二抗
Substrates for assay procedure取决于二抗
TagStrep-tag
Epitope detectedSAWSHPQFEK

Resources

试剂盒操作手册 (1)
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)
Kit Handbooks (1)

FAQ

What is the basic technology behind the Strep-tag Protein Purification System?

The basic technology behind the Strep-tag System is a Two-Step Affinity Protein Purification using sequential purification of recombinant proteins carrying two affinity tags. Proteins that carry these two small tags (the 6xHis tag and the 8 amino acid containing Strep-tag) are efficiently expressed in E. coli, insect, or mammalian cells using pQE-TriSystem His·Strep vectors. After cell lysis and clearing of the lysate, proteins are initially purified by a procedure based on the proven 6xHis-tag-Ni-NTA interaction. After elution from the Ni-NTA matrix using imidazole, recombinant proteins (which also carry the Strep-tag epitope) are loaded directly onto a Strep-Tactin matrix (Strep-Tactin SuperFlow or Strep-Tactin Magnetic Beads). Protein is eluted from the Strep-Tactin matrix using either biotin or desthiobiotin. This two-step affinity purification delivers ultrapure (>98% pure) protein. The order of purifications can also be reversed (i.e., Strep-Tactin followed by Ni-NTA purification). Since purification is done under native conditions, the Two-Step Affinity Purification System can be beneficial for highly demanding applications (i.e., crystallization studies).

 

FAQ ID -740
What is the concentration of SureENTRY Transduction Reagent?
The concentration of SureENTRY Transduction Reagent is 10 mg/ml.
FAQ ID - 3708