S_1335_PROT_QXP0554

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Penta·His HRP Conjugate Kit

Cat. No. / ID:  34460

125 μl Penta·His HRP Conjugate,5 g 封闭试剂,50 ml 封闭试剂缓冲液(10x 浓缩液)
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Kit
Penta·His HRP
RGS·His HRP
Anti·His HRP Conjugate Kits 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • His 标签蛋白(也具有 RGS·His 表位)的高灵敏度和高速检测
  • 无需使用二抗
  • 提供经过优化的封闭试剂

Product Details

Anti·His HRP Conjugate Kit,包括 RGS·His HRP Conjugate Kit(His 标签轭合物)和 Penta·His HRP Conjugate Kit(6xHis 标签轭合物),旨在用于携带 His 标签的重组蛋白的灵敏性检测,而无需使用二抗。它们由与辣根过氧化物酶 (horseradish peroxidase, HRP) 结合的抗 His 抗体组成。在检测程序中,抗 His 抗体与蛋白质的 His 标签结合,然后使用与 HRP 发生反应的化学发光或显色底物定位复合物。

Performance

特点RGS·His HRP Conjugate KitPenta·His HRP Conjugate Kit
检测N-末端、C-末端和内部 His 标签(包含 RGSHHHH 表位)N-末端、C-末端和内部 6xHis 标签
斑点印迹分析中的灵敏度*10 pg10 pg
蛋白质印迹分析中的灵敏度*50 pg50 pg
抗体同种型小鼠 IgG1小鼠 IgG1
交叉反应与天然 E. coli、酵母、哺乳动物或昆虫细胞裂解物的交叉反应可忽略不计与天然 E. coli、酵母、哺乳动物或昆虫细胞裂解物的交叉反应可忽略不计
剂型稳定的原液稳定的原液
工作溶液125–250 ml125–250 ml

Principle

Anti·His HRP Conjugate Kit 由与报告酶辣根过氧化物酶 (horseradish peroxidase, HRP) 化学偶联的 QIAexpress 抗 His 抗体组成。它们可用于对任何具有可接近 His 标签的重组蛋白进行高度特异性和敏感性直接检测。它们消除了对二抗的需求,从而节省了免疫检测步骤的时间。Anti·His HRP Conjugate Kit 可与化学发光或显色检测底物配合使用。

Procedure

随所有 QIAexpress 检测产品一起提供的《QIAexpress 检测手册》包含用于设计和执行检测的详细示例方案和指南。

Applications

Anti·His HRP Conjugate Kit,包括 RGS·His HRP Conjugate Kit(His 标签偶联物)和 Penta·His HRP Conjugate Kit(6x His 标签偶联物),能够识别与其相应抗体相同的表位,并可在下列应用中用于 His 标签蛋白的直接化学发光或显色检测:

  • 蛋白质、斑点和菌落印迹分析
  • ELISA 和其他免疫检测分析

RGS·His HRP Conjugate Kit 与 Penta·His HRP Conjugate Kit 的比较

特点 RGS·His HRP Conjugate Kit Penta·His HRP Conjugate Kit
结合的酶 HRP HRP
检测 需要二抗 需要二抗
检测的表位 RGSHHHH HHHHH
用于印迹检测的底物 Biotech ECL 4C1N
用于检测程序的底物 OPD OPD
标签 His 标签 His 标签

Anti·His HRP Conjugate Kit 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。

Resources

安全数据表 (2)
Download Safety Data Sheets for QIAGEN product components.
Download Safety Data Sheets for QIAGEN product components.
试剂盒操作手册 (1)
For Anti·His Antibodies, Anti·His HRP Conjugates, Penta·His™ Alexa Fluor® Conjugates, Penta·His Biotin Conjugate, Ni-NTA Conjugates, Tag·100™ Antibody, Streptavidin–R-PE, 6xHis Protein Ladder, Ni-NTA HisSorb™ Strips and Plates 
Certificates of Analysis (1)
Kit Handbooks (1)
For Anti·His Antibodies, Anti·His HRP Conjugates, Penta·His™ Alexa Fluor® Conjugates, Penta·His Biotin Conjugate, Ni-NTA Conjugates, Tag·100™ Antibody, Streptavidin–R-PE, 6xHis Protein Ladder, Ni-NTA HisSorb™ Strips and Plates 
Supplementary Protocols (1)

FAQ

What epitopes do the Anti-His Conjugates and Anti-His Antibodies recognize?

The table below lists the epitopes recognized by the Anti-His Conjugates and Anti-His Antibodies. 'H' designates a histidine residue, while 'X' designates any amino acid other than histidine. Note that individual 6xHis-tagged proteins are often recognized better by one Anti-His Antibody than by the others, possibly because of subtle differences in the exact conformation of the 6xHis tag and other parts of the protein in the vicinity of the tag. To choose the optimal antibody for your specific 6xHis-tagged protein and application, QIAGEN offers the Anti-His Antibody Selector Kit. The kit includes 3 µg of each of the three antibodies for direct comparison and economical selection of the one that gives the best results.

Epitopes recognized by QIAexpress Anti-His HRP Conjugates and Anti-His Antibodies


Peptide Penta-His Tetra-His RGS-His
XHHHHHHX
+ +
XXHHHHHX
+ +
XHXHHHHX
+
XHHHHXHX
+
XRGSHHHHX
+ +
XRGSHHHHHHX
+ + +
XHXHHXHX

Note that the presence of specific residues N- or C-terminal to the epitope is not required.

FAQ ID -170
Which Anti-His Antibody is the most sensitive for my protein of interest?

Penta-His and Tetra-His Antibody bind to proteins that have 6xHis tags regardless of the surrounding amino-acids, even when the tag is partially hidden. Subtle differences in the antigen recognition sites of these antibodies mean that some 6xHis-tagged proteins are detected with higher sensitivity by one Anti-His Antibody than by the others (see figure below). Therefore, to achieve optimum results, we recommend comparison of these antibodies in parallel for new applications and proteins. QIAGEN offers the Anti-His Antibody Selector Kit for this purpose.


 

Sensitivity of QIAexpress Anti·His Antibodies



 

Note:  RGS His Antibody regonizes the RGS(His)4 epitope that is found on proteins endcoded by most pQE vectors as well as several pRSETan pBlue BacHis -vectors from Invitrogen.

FAQ ID -172
How can I avoid poor immunolocalization morphology with Anti-His Antibodies?
Insufficient fixation of samples leads to poor morphological preservation. If using methanol or acetone try fixation with paraformaldehyde. If 2% paraformaldehyde is insufficient, increase fixative concentration to 4% and increase incubation time. Keep in mind that too harsh fixation can lead to functional alteration of the 6xHis epitope and loss of recognition by the Anti·His Antibodies.immunofluorescence
FAQ ID -200
Can the Blocking Reagent supplied with the Penta-His HRP Conjugate be used with other antibodies?
Yes, the Blocking Reagent supplied with our Penta-His HRP Conjugate can also be used with other antibodies.
FAQ ID -1073