RT² PreAMP cDNA Synthesis Kit

从1–100 ng RNA中预扩增cDNA，用于RT² Profiler PCR Arrays分析

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RT² PreAMP cDNA Synthesis Kit (12)

Cat. No. / ID: 330451

RT² Nano PreAMP cDNA Synthesis Kit

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RT² PreAMP cDNA Synthesis Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

使用ng级RNA进行预扩增
可扩增89个基因特异性目标cDNA
在下游PCR分析中具有较少的非特异性反应

Product Details

RT² PreAMP cDNA Synthesis Kit可从ng级（1–100 ng）RNA中预扩增cDNA，专用于RT² Profiler PCR Arrays分析。

Principle

RT² PreAMP技术利用多重、串联PCR对基因特异性cDNA进行最小偏向性的预扩增。RT² PreAMP cDNA Synthesis Kit可从1–100 ng总RNA中预扩增cDNA。

标准逆转录后，在使用RT² Profiler PCR Array进行基因表达分析前，可使用RT2 PreAMP Pathway Primer Mix从cDNA中预扩增芯片特异性靶标。每个RNA样本可产生足够多的cDNA，可用于至多4个不同RT² Profiler PCR Arrays的基因表达分析。

Procedure

操作流程包括2个简单的步骤：

合成cDNA：该试剂盒可用于从至多12个不同的RNA样本中合成cDNA。RT² Profiler PCR Arrays的逆转录对照（RTC）可检测内置的RNA外参模板，用于检测逆转录抑制剂，确保高效合成第一条链。
预扩增通路特异性基因的cDNA：从1–100 ng总RNA中合成的每个cDNA，都可用至多4组不同的PCR芯片特异性引物混合物进行扩增，用于至多4个不同RT² Profiler PCR Arrays的基因表达分析。试剂盒中的Side Reaction Reducer可去除预扩增时残留的引物，保证PCR芯片的准确检测。

为完成PCR芯片分析，预扩增的模板需与仪器专用的即用型RT² SYBR^® Green qPCR Mastermix混合。

Applications

使用RT² PreAMP cDNA Synthesis Kit和RT² PreAMP Pathway Primer Mixes获得的cDNA模板可直接用于RT² Profiler PCR Arrays基因表达谱分析。

Resources

Download Safety Data Sheets for QIAGEN product components.

Simultaneously profile mRNA, miRNA and lncRNA using a simple, complete workflow

FAQ

Yes, stained sections can be used with the RT² PreAMP cDNA Synthesis Kit.

FAQ ID -2722

For best results, all RNA samples should be suspended in RNase-free water. Alternatively, RNase-free 1 mM sodium citrate (pH 6.5) or 10 mM Tris buffer (pH 7.0) may be used. Do not use DEPC-treated water, as most DEPC preparations are contaminated with molecules that are inhibitory to reverse transcription and/or PCR. For long-term storage, RNA preps may be stored at -70 ºC in RNase-free water, or the buffers listed above, or precipitated in ethanol or isopropanol. In order to avoid repeated freeze-thaw cycles, it is recommended that frozen RNA samples be stored as multiple, single-use aliquots.

FAQ ID -2659

RT2 PreAMP cDNA Synthesis Kit

从1–100 ng RNA中预扩增cDNA，用于RT2 Profiler PCR Arrays分析