S_1202_IAS_RGQ_0083_s

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Rotor-Gene Q 2plex Priority Package Plus

Cat. No. / ID:  9001862

Real-time PCR cycler with 2 channels (green, yellow), laptop computer, software, accessories: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
Copy order details
InstrumentSoftware
Rotor-Gene Q
Rotor-Gene Q HRM
Rotor-Gene ScreenClust HRM Software
Multi-channel detection
2plex
5plex
6plex
Service Options
Priority Package Plus
Priority Package
System
Platform
Inquire
Rotor-Gene Q实时荧光定量PCR分析仪应与相应的QIAGEN试剂盒配合使用,适用于试剂盒用户手册中指定的应用。如果将Rotor-Gene Q实时荧光定量PCR分析仪与非QIAGEN的试剂盒配合使用,用户应自行确认是否适用于相关应用。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 转子式设计,确保卓越的热力学和光学性能
  • 无与伦比的光学范围,波长从紫外到红外
  • 界面友好的软件,支持领先的分析
  • 强大的设计,确保最低的维护需求及最大的方便性
  • 配合QIAGEN分析技术,确保结果可靠的多重应用

Product Details

QIAGEN的Rotor-Gene Q实时荧光定量PCR分析仪结合多种优化的设计特性,具有卓越的性能,确保结果可靠。配合经优化的QIAGEN real-time PCR试剂盒,Rotor-Gene Q实时荧光定量PCR分析仪可进行简单的分析,用于多种领域。

Performance

Rotor-Gene Q实时荧光定量PCR分析仪是目前唯一一台可通过HRM分析最高难度级,IV级别SNPs的real-time PCR仪。QIAGEN专为您提供HRM试剂盒,用于HRM的各种应用,如基因分型(参见" Identification of a class IV SNP" for data from the Type-it HRM PCR Kit),定量甲基化分析(参见" Highly sensitive detection of methylated DNA" for data from the EpiTect HRM PCR Kit),基因扫描和序列匹配。Type-it HRM PCR Kit支持可靠、准确基因突变和SNP的检测。EpiTect HRM PCR Kit可实现快速筛查和精确检测亚硫酸氢盐转化后DNA的CpG甲基化水平的变化。
See figures

Principle

独特的转子设计,卓越的性能

Rotor-Gene Q实时荧光定量PCR分析仪独特的离心转子式设计使其成为当前最精确的多功能定量PCR仪(参见" Cross-section of the Rotor-Gene Q")。 反应仓中各离心管在快速热循环过程中能够保持相同的温度,光学检测具有同样的均一性。每个试管与检测光成一线时,会激发样本,并通过单个短光路快速收集到荧光信号。均一的热性能和光学性能确保灵敏和准确的定量PCR分析(参见" Precise real-time PCR analysis")。 避免了传统板式PCR仪无法避免的样本间差异及边缘效应。

转子式设计确保下列优势:

  • 管间温差小于±0.02°C
  • 均一检测,无需使用ROX校正染料
  • 快速升降温和可忽略不计的平衡时间,缩短了反应时间
  • 完全可靠的结果
无与伦比的光学范围,适合多种应用

无论分析是基于如SYBR® Green的内参染料、如水解(TaqMan)的探针法还是杂交(FRET)探针、Scorpion探针,或多种化学试剂,Rotor-Gene Q实时荧光定量PCR分析仪都能满足所有需求。该仪器提供多至6个通道,波长从紫外到红外,是当前光谱范围最广的PCR仪(见表)。此外,应用相应的软件可获得新的激发/检测光波长组合,这就意味着Rotor-Gene Q实时荧光定量PCR分析仪与将来可能使用的染料兼容。

光学检测通道
通道 激发(nm) 检测(nm) 适用的荧光素举例
365 ± 20 460 ± 20 Marina Blue, Edans, Bothell Blue,
Alexa Fluor 350, AMCA-X
绿 470 ± 10 510 ± 5 FAM, SYBR® Green I, Fluorescein,
EvaGreen, Alexa Fluor 488
530 ± 5 557 ± 5 JOE, VIC, HEX, TET, MAX, CAL Fluor Gold 540, Yakima Yellow
585 ± 5 610 ± 5 ROX, CAL Fluor Red 610, Cy 3.5,
Texas Red, Alexa Fluor 568
625 ± 5 660 ± 10 Cy5, Quasar 670, LightCycler
Red640, Alexa Fluor 633
深红 680 ± 5 712 high pass Quasar 705, LightCycler Red705,
Alexa Fluor 680
HRM 460 ± 20 510 ± 5 SYBR® Green I, SYTO9, LC Green,
LC Green Plus+, EvaGreen
使用HRM,扩展您的研究

High-resolution melting analysis (HRM)作为一种闭管式的PCR后分析技术,引起了学术界的广泛关注。HRM基于DNA双链的熔解特性对PCR产物进行分析,与传统的熔解曲线分析类似,但提供更多的信息,因而具有更广泛的应用。可根据PCR产物的序列、长度、GC含量或链互补性以及单个碱基对的变化来区分,其灵敏度可区分单个碱基对的差别。现在可以通过这种高效、简单的方法检测、分析未知的甚至复杂的序列。Rotor-Gene Q实时荧光定量PCR分析仪的转子式设计和出色的热性能及光学表现使其高度适用于HRM。

Rotor-Gene Q实时荧光定量PCR分析仪的HRM选项包括:

  • 特殊调校的高强度HRM光学通道
  • 温度分辨率为0.02°C
  • 快速采集数据
  • 功能全面的HRM软件
最少的维护需求,最大的方便性

Rotor-Gene Q实时荧光定量PCR分析仪的设计降低了维护需求,使用方便。节约时间和成本,让您可以将更多精力集中于科研工作,而不是围着机器。

Rotor-Gene Q实时荧光定量PCR分析仪的方便性如下:

  • 高度稳定的LED光源,保证其寿命,无需更换光源或激光器,避免光学性能逐渐降低
  • 安装时及仪器移动后无需光学校正
  • 无需清洁孔板
  • 不存在旋转引起的冷凝和气泡
  • 小巧、轻便、功能强大,可随意放置仪器
See figures

Procedure

灵活的规格,满足您的需求

Rotor-Gene Q实时荧光定量PCR分析仪支持多种规格的PCR管,满足各种需求。只需更换承载PCR管的金属转子,便可轻松更换规格。除了单管,也提供加快反应体系构建、提高通量的Rotor-Disc。Rotor-Disc是环状板,反应孔为垂直方向。Rotor-Disc 100相当于一个96孔板加4个参照管。这些多出的反应孔可用于更多反应或参照。也提供72孔的Rotor-Disc 72。使用Rotor-Disc Heat Sealer可快速方便的用塑料膜密封Rotor-Disc。所有需要应用Rotor-Disc运行的反应,选择Rotor-Disc 100 Starter Kit或Rotor-Disc 72 Starter Kit。

可手动构建反应体系,或使用QIAGEN自动化方案自动构建反应体系。QIAgility可经济、快速、高速准确的构建反应体系,同时QIAsymphony AS是进行常规日间PCR差异检测的理想选择。所有仪器可在Rotor-Gene模式下自动构建反应体系,可直接传递样本清单,并为各种不同的实验方案提供定量PCR预混液。

简单的常规验证

实验室经常需要进行常规的性能精确性验证。对于大部分的PCR仪,这个过程需要工程师的介入。而对于Rotor-Gene Q实时荧光定量PCR分析仪,这不是必需的。使用简单、价格合理的Rotor-Disc OTV(Optical Temperature Verification)Kit可自动进行精确性检测。几分钟内完成整个流程。

Applications

一系列可用于Rotor-Gene Q实时荧光定量PCR分析仪的QIAGEN试剂盒能够在所有定量PCR应用中可靠定量,无需优化反应和循环条件。用于定量PCR和HRM应用的试剂盒,适合多种应用:

  • 基因表达分析 
  • 病原体检测
  • 基因分型和基因扫描
  • miRNA研究

Software

软件支持定量,提高数据的安全性

功能全面的Rotor-Gene Q实时荧光定量PCR分析仪软件包支持当前领先的定量分析流程,涵盖了所有基本和高级的数学运算法则。您可以完全自主地分析宝贵的数据,提高结果的可靠性。软件确保数据安全,从运行开始到结果输出全程都可追踪。

更先进的软件,采用HRM分析用于基因分型和突变检测

Rotor-Gene ScreenClust HRM Software是Rotor-Gene运行软件的扩展。该软件是当前功能较强大的工具,适合分析从Rotor-Gene Q实时荧光定量PCR分析仪或Rotor-Gene 6000循环仪获得的HRM数据。对于一组样本的分析,Rotor-Gene ScreenClust HRM Software打开了一个新的模块,用于如基因分析和突变检测等HRM分析应用。

Services

Have your throughput demands increased? Do you want to expand your application range or further streamline workflows? Do you need to manage more complex samples? Do you need improved connectivity?

If the answer to any of these questions is yes, then QIAGEN’s trade-in/trade-up program is just right for you!

QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply exchange an old instrument for a new one. Improve your efficiency by trading in a competitor instrument or trading up with an older QIAGEN instrument.

Learn more about trade-in/trade-up opportunities.

Supporting data and figures

Specifications

FeaturesSpecifications
DimensionsWidth, 37 cm (14.6 in.); Height, 28.6 cm (11.3 in.); Depth (without cables), 42 cm (16.5 in.); Depth (door open), 53.8 cm (21.2 in.)
Operating temperature18-30ºC (64-86ºF)
Pollution level2; Environmental class 3K2 (IEC 60721-3-3)
Humidity10–75% (noncondensing)
FeaturesDynamic range, 10 orders of magnitude
Protocols/main application on this instrumentGene expression analysis, microRNA detection, Virus detection, SNP genotyping, SNP genotyping, High Resolution Melt analysis (HRM)
SoftwareRotor-Gene Q software, supplied on the installation CD provided
Overvoltage categoryII
Heat dissipation/thermal loadAverage, 0.183 kW (632 BTU/hour); Peak, 0.458 kW (1578 BTU/hour)
Optical SystemUp to 6 channels spanning UV to infra-red wavelengths; Excitation sources: High energy light-emitting diodes; Detector: Photomultiplier; Acquisition time: 4 s. The software allows to create new excitation/detection wavelength combinations
Power100–240 V AC, 50–60 Hz, 560 VA (peak); Power consumption 8 VA (standby); Mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages; F5a 250 V fuse
Kits designed for this instrumentartus QS-RGQ Kits (not available in all countries), RG SYBR Green PCR Kits; RG SYBR Green RT-PCR Kit; RG Probe PCR Kits; RG Probe RT-PCR Kit; RG Multiplex PCR Kit
Place of operationFor indoor use only
Samples per run (throughput)Tubes 0.2 ml; Strip Tubes 0.1 ml (4 tubes); Rotor-Disc 72; Rotor-Disc 100; Up to 100 samples per run using a Rotor-Disc 100
Storage conditions15ºC to 30ºC (59ºF to 86ºF) in manufacturer’s package; Max. 75% relative humidity (noncondensing); Environmental class 1K2 (IEC 60721-3-1)
TechnologyReal-time PCR cycler
Transportation conditions–25ºC to 60ºC (–13ºF to 140ºF) in manufacturer’s package; Max. 75% relative humidity (noncondensing); Environmental class 2K2 (IEC 60721-3-2)
Warranty1 year on instrument; lifetime guarantee on excitation LEDs
Thermal performanceTemperature range, 35 to 99ºC; Temperature accuracy, ±0.5ºC (type, measured 30 seconds after clock start); Temperature resolution, ±0.02ºC (smallest programmable increment); Temperature uniformity, ±0.02ºC; Ramp rate (peak ramp rates, air), >15ºC/s heating, >20ºC/s cooling
Typical run time40 cycles in 45 min with the QIAGEN RG Kits (assay dependent)
Weight12.5 kg (27.6 lb.), standard configuration
AltitudeUp to 2000 m (6500 ft)

Service Plans

RGQ, Full Agreement

Cat. No. / ID:  9242698

Instrument repair service for the Rotor-Gene Q at regional repair center. Shipping, labor and parts included during the Full Agreement period. Loaner instrument provided within 2–3 business days. Instrument repair turnaround time of 7–10 business days. Includes one on-site Preventive Maintenance or Inspection Service during the Full Agreement period.
Copy order details
RGQ, Full Agreement, no PM

Cat. No. / ID:  9241781

Instrument repair service for the Rotor-Gene Q at regional repair center. Shipping, labor and parts costs included during the Full Agreement period. Loaner instrument provided within 2–3 business days. Instrument repair turnaround time of 7–10 business days.
Copy order details
RGQ, Core Agreement

Cat. No. / ID:  9245253

One-time Rotor-Gene Q instrument repair performed at a regional repair center including shipping, labor, and spare parts for a period of one year. Instrument repair turnaround time of 7–10 business days. Includes one on-site Preventive Maintenance or Inspection Service and a 10% discount on additional repair service during the Core Agreement period.
Copy order details

Resources

快速启动实验方案 (1)
分析软件 (8)
For use with the Q-Rex Software for determination of genotypes using High-Resolution Melting analysis.
For use with the Q-Rex Software to classify the genotypes of unknown samples.
Q-Rex Basic Plug-in 3.0.0
SOFTWARE (1MB)
For use with the Q-Rex Software to visualize fluorescence data and determine quantification cycle values based on an automatic user independent threshold algorithm.
Gene Expression Analysis Plug-in for the Q-Rex Software
For use with the Q-Rex Software v1.0 or higher to detect targets
For use with the Q-Rex Software or higher to calculate absolute concentration of targets by PCR. The plug-in is dedicated to our customers 
For use with the Q-Rex Software to calculate absolute concentration of targets by PCR
软件使用指南 (9)
For use with Q-Rex Software version 1.0 to calculate absolute concentration of targets by PCR. This user manual provides information about the functions and features of the Q-Rex Absolute Quantification HID plug-in.
Gene Expression Plug-in v3.0; for use with Q-Rex Software v2.0 for Rotor-Gene Q and QIAquant to perform relative quantitation analyses
Target detection Plug-in v2.0; for use with the Q-Rex Software v2.0 for Rotor-Gene Q and QIAquant to detect targets by PCR
For use with the Q-Rex Software v1.0 for determination of genotypes using High-Resolution Melting analysis. This user manual provides information about the functions and features of the Q-Rex HRM Analysis plug-in.
For use with the Q-Rex Software v2.0 for Rotor-Gene Q and QIAquant to classify the genotypes of unknown samples
Absolute Quantification Plug-in v3.0; for use with the Q-Rex Software v2.0 for Rotor-Gene Q and QIAquant to calculate absolute concentration of targets by PCR
For use with the Q-Rex Software v2.0 for Rotor-Gene Q and QIAquant for determination of genotypes using Melt Curve Analysis
Basic Plug-in v3.0; for use with the Q-Rex Software v2.0 for Rotor-Gene Q and QIAquant to determine quantification cycle values
For use with the Rotor-Gene Q and QIAquant instruments
产品介绍与指南 (4)
Second edition — innovative tools
Now with even more applications!

Streamline your workflow with QIAGEN solutions for human identity and forensic testing

用户使用手册 (7)
For Rotor-Gene Q instruments using Rotor-Gene Q Software version 2.3.4
Guideline for ISO 20836:2020
For use with Rotor-Gene Q instruments and Q-Rex Software version 1.0
For use with Rotor-Gene Q Software version 2.3.4
Alternative options for the optical drive (DVD-ROM drive) on the Rotor-Gene Q Notebook, Part Number 9026760
操作软件 (4)
For use with Q-Rex Software to import sample information from QIAgility instruments
Q-Rex Software 2.0.0
SOFTWARE (47MB)

For use with the Rotor-Gene Q and QIAquant instruments.

Note: The Q-Rex Software is compatible only with QIAquant instruments with serial numbers that start with 3108 (produced after 11/2021).
For use on the Rotor-Gene Q. Rotor-Gene Q software 2.3.5 is compatible with Windows 7 and Windows 10 operating systems
For use with the Q-Rex Software or higher to create new experiments quickly and easily based on existing QIAGEN kit templates.
试剂盒操作手册 (3)
For verification of thermal accuracy of Rotor-Gene real-time cyclers
Safety Data Sheets (1)
Certificates of Analysis (1)
Brochures & Guides (4)
Second edition — innovative tools
Now with even more applications!

Streamline your workflow with QIAGEN solutions for human identity and forensic testing

Kit Handbooks (3)
For verification of thermal accuracy of Rotor-Gene real-time cyclers
Instrument User Manuals (7)
For use with Rotor-Gene Q Software version 2.3.4
For Rotor-Gene Q instruments using Rotor-Gene Q Software version 2.3.4
Guideline for ISO 20836:2020
Alternative options for the optical drive (DVD-ROM drive) on the Rotor-Gene Q Notebook, Part Number 9026760
For use with Rotor-Gene Q instruments and Q-Rex Software version 1.0
Quick-Start Protocols (1)
Analysis Software (8)
For use with the Q-Rex Software or higher to calculate absolute concentration of targets by PCR. The plug-in is dedicated to our customers 
For use with the Q-Rex Software to calculate absolute concentration of targets by PCR
Q-Rex Basic Plug-in 3.0.0
SOFTWARE (1MB)
For use with the Q-Rex Software to visualize fluorescence data and determine quantification cycle values based on an automatic user independent threshold algorithm.
For use with the Q-Rex Software to classify the genotypes of unknown samples.
Gene Expression Analysis Plug-in for the Q-Rex Software
For use with the Q-Rex Software for determination of genotypes using High-Resolution Melting analysis.
For use with the Q-Rex Software v1.0 or higher to detect targets
Operating Software (4)
For use on the Rotor-Gene Q. Rotor-Gene Q software 2.3.5 is compatible with Windows 7 and Windows 10 operating systems
Q-Rex Software 2.0.0
SOFTWARE (47MB)

For use with the Rotor-Gene Q and QIAquant instruments.

Note: The Q-Rex Software is compatible only with QIAquant instruments with serial numbers that start with 3108 (produced after 11/2021).
For use with the Q-Rex Software or higher to create new experiments quickly and easily based on existing QIAGEN kit templates.
For use with Q-Rex Software to import sample information from QIAgility instruments
Software User Guides (9)
For use with the Rotor-Gene Q and QIAquant instruments
Absolute Quantification Plug-in v3.0; for use with the Q-Rex Software v2.0 for Rotor-Gene Q and QIAquant to calculate absolute concentration of targets by PCR
Gene Expression Plug-in v3.0; for use with Q-Rex Software v2.0 for Rotor-Gene Q and QIAquant to perform relative quantitation analyses
Target detection Plug-in v2.0; for use with the Q-Rex Software v2.0 for Rotor-Gene Q and QIAquant to detect targets by PCR
For use with Q-Rex Software version 1.0 to calculate absolute concentration of targets by PCR. This user manual provides information about the functions and features of the Q-Rex Absolute Quantification HID plug-in.
Basic Plug-in v3.0; for use with the Q-Rex Software v2.0 for Rotor-Gene Q and QIAquant to determine quantification cycle values
For use with the Q-Rex Software v1.0 for determination of genotypes using High-Resolution Melting analysis. This user manual provides information about the functions and features of the Q-Rex HRM Analysis plug-in.
For use with the Q-Rex Software v2.0 for Rotor-Gene Q and QIAquant to classify the genotypes of unknown samples
For use with the Q-Rex Software v2.0 for Rotor-Gene Q and QIAquant for determination of genotypes using Melt Curve Analysis

FAQ

Which-reporter-dyes-can-be-combined-for-use-in-multiplex-PCR-on-the-Rotor-Gene-Q-Cycler?
Please refer to section 'Guidelines for effective multiplex assays' under "Important Notes" in the Rotor-Gene Multiplex PCR Handbook for suitable combinations of reporter dyes, or visit our Multiplex real-time PCR Resource site for additional information.
FAQ ID -9028
Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC?

Based on our extensive and successful testing of many QuantiTect Primer Assays with Rotor-Gene SYBR Green PCR Kits, we guarantee this.

 

 

FAQ ID -2124
What reaction volume is suitable for use in the Rotor-Gene Q?

Reaction volumes suitable for use on the Rotor-Gene Q are:

  • Rotor-Disc 100: 30 µl x 100-wells, 10-25 µl reaction volume
  • Rotor-Disc 72: 0.1 ml x 72-wells, 15-25 µl reaction volume
  • Strip Tubes 0.1 ml: 0.1 ml x 72-wells, 10-30 µl reaction volume, strips of 4 tubes and caps
  • PCR Tubes 0.2 ml: 0.2 ml x 36-wells, 15-50 µl reaction volume, individual tubes with caps
FAQ ID -9030
If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes?

The Rotor-Gene Q software allows creation of new excitation/detection wavelength combinations, which means that Rotor-Gene Q will work with dyes you may want to use in the future.

 

FAQ ID -2087
Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits?
What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software?

'Probability' is the likelihood or chance that a sample is a member of each available cluster. The combined probabilities of a single sample add up to 1.00.

'Typicality' in the Rotor-Gene ScreenClust HRM analysis is a measure of how well a sample fits into its assigned cluster. It can also be seen as a measure of how far away a sample is from the centre of the cluster. Typicality values range from 0 to 1, the higher the value the closer it is to the centre of its assigned cluster. If a sample has a typicality value of 0.5, it means that approximately half of all samples within that cluster will be closer to the centre and the other half will be further away. In reality, this might not be the case as small samples numbers can have skewed distributions.

 

FAQ ID -2203
Which downstream applications have been tested with SARS-CoV-2-derived RNA purified from saliva collected into PAXgene Saliva Collector?

RNA purified with the QIAamp Viral RNA Mini Kit has been used for quantification by qPCR with QuantiTect Probe RT-PCR Kit on QIAGEN Rotor-Gene Q.

3828
Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?

Clusters in Rotor-Gene ScreenClust HRM Software are graphically represented by ellipses/ellipsoids which act as a visual aid. They are not designed to cover all of the samples. They are a good tool for compare differences between clusters. To judge how well individual samples fit within their clusters use the typicality scores.

 

 

FAQ ID -2204
Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?

The controls define the centre of the clusters in supervised mode using Rotor-Gene ScreenClust HRM Software. If the control samples lie on the fringe of the cluster then the cluster centre can be shifted away from the bulk of the samples within the cluster. Controls should provide a good representation of the expected behavior of unknown samples. If this is not the case the experimental setup should be re-evaluated.

 

FAQ ID -2205
How is the hypothesis test performed in the REST software?
In the REST 2009 software, the hypothesis test performs 10,000 random reallocations of samples and controls between the groups, and counts the number of times the relative expression on the randomly assigned group is greater than the sample data.
FAQ ID -2459
How do Rotor-Gene Probe Kits compare with QuantiFast Probe Kits?

Rotor-Gene Probe Kits are specially developed for Rotor-Gene cyclers. The unique rotary system of the cyclers combined with the kits’ proprietary buffer system enable ultrafast cycling. Rotor-Gene Probe Kits do not contain ROX dye.

 

FAQ ID -2125
Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled?

Both types of tubes run on the Rotor-Gene Q, and can be labeled on the top without any interference with data acquisition, because signal is detected from the bottom of the tube. Labeling is helpful and can prevent possible sample mix up.

 

FAQ ID -2082
Does the Rotor-Gene Q software have the capacity to export data?

There are several features available to allow exporting data across platforms to a variety of software packages. Sample names can easily be imported into the Rotor-Gene Q Excel sample sheets. By using a function "Export to Excel" the software automatically exports any results table in the software to Excel. Furthermore, reports can now be exported as Word files, which make the data compatible with any Microsoft office/Mac application. Reports can also be saved or sent in HTML format. All figures and graphs can be exported as Jpeg or Bitmap files for use in any desktop publishing software.

 

FAQ ID -2089
Why do I need normalization using Rotor-Gene ScreenClust HRM Software?

Normalization using Rotor-Gene ScreenClust HRM Software is required since HRM melt curves can have different starting points. Therefore the scale of each melt curve can be different. Comparison can only occur if all samples are on the same scale, so each curve needs to be normalized.

 

FAQ ID -2198
Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment?

No. The master mixes in Rotor-Gene Kits contain dTTP instead of dUTP. If UNG treatment is required, we recommend using QuantiTect +UNG Kits. QuantiTect Kits are also compatible with the Rotor-Gene Q; however, the kits require a significantly longer cycling time.

 

 

FAQ ID -2117
In the REST software, what is the range indicated by 'Std. Error' on the 'Results' page?
In the REST 2009 software, the range for the Standard Error on the results tab is 68% C.I. (confidence interval).
FAQ ID -2455
Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results?

No. The fixed optical path ensures uniform illumination and detection from sample to sample eliminating the need to use a reference dye such as ROX on the Rotor-Gene Q.

 

 

FAQ ID -2079
Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run?

Yes. The sample sheet can be amended during or after a PCR run by activating the 'Edit samples' button in the Rotor-Gene Q software. The sample sheet will open and may be modified.

 

FAQ ID -2092
Can data be analyzed on the Rotor-Gene Q while a run is in progress?

Yes, data can be analyzed on the Rotor-Gene Q while a run is in progress, allowing to save time for planning and setting up new experiments. Multiple copies of the Rotor-Gene Q software can be opened simultaneously during a run allowing analysis of previous experiments while the system is running.

 

FAQ ID -2091
What is a QuantiTect Primer Assay?

QuantiTect Primer Assays are primer pairs designed and bioinformatically validated specifically for real-time RT-PCR with SYBR Green detection. To find a primer assay for your target gene of interest, please visit our GeneGlobe data base.

For best results, we strongly recommend using QuantiTect Primer Assays in combination with QIAGEN's products for SYBR Green-based Real-Time PCR and RT-PCR.

FAQ ID -1141
Why is no fluorescence signal detected in my run?

Please make data are collected in the appropriate fluorescent channel. Also check the gain is optimized.

If the issue persists, please send the original run file with extension .rex to QIAGEN Technical Service for further assistance.

FAQ ID -9023
If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software?

The reaction volume to be entered in the Rotor-Gene Q software should be the sum of the PCR reaction volume and the volume of Vapor-Lock.

 

 

FAQ ID -2150
How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q?

At the selected data acquisition step of the thermal cycle on the Rotor-Gene Q, the fluorescent signal intensity is measured 20 times for each sample as they spin past the detector. Tubes on a rotor spin past the excitation/detection optics every 150 milliseconds. The average of the 20 readings is then taken as the fluorescence of the sample at that cycle number.

 

FAQ ID -2083
What should I do if the Rotor-Disc OTV run does not pass?

Please send the original OTV run file to QIAGEN Technical Service for further assistance.

FAQ ID -9022
Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?

This type of cycling allows a significant reduction in cycling time for Rotor-Gene SYBR Green PCR Kits. It is more effective than reducing the individual times for annealing and extension.

 

FAQ ID -2122
What types of reaction vessels are required for use in the Rotor-Gene Q?

We strongly recommend use of the dedicated Rotor-Gene Q Accessories, e.g., PCR Tubes 0.2 ml or Strip Tubes and Caps 0.1 ml provided by QIAGEN. These tubes are designed to give low background and perfectly match the Rotor-Gene Q requirements.

 

FAQ ID -2085
Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?

The buffer composition, which affects the initial reactivation of HotStarTaq Plus DNA Polymerase, has been optimized for each respective Rotor-Gene Kit.

 

FAQ ID -2118
How long does a run take?
An Investigator Quantiplex Pro or Quantiplex Pro RGQ run of 40 cycles takes ca. 60 minutes. 
For Investigator Quantiplex on the Rotor-Gene Q, a run of 40 cycles takes ca. 55 minutes. On the Applied Biosystems 7500 Real-Time PCR System, a run of 40 cycles takes ca. 57 minutes.
FAQ ID -2566
How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?

The Rotor-Gene Q instrument can be used with two different rotor formats: using tubes or rotor-dics. Tubes can be run in 36 and 72-well rotors and rotor-discs in Rotor-Disc 72 and Rotor-Disc 100 rotors. When programming the temperature profile please make sure the correct rotor type is selected.  

 

 

FAQ ID -1519
What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software?

Principal component analysis is a well-established method of data analysis for multivariate data sets, such as obtained from, e.g.,  microarray analysis or image analysis. However, it is new in ScreenClust for HRM data. Principal Components (PCs) are extracted from the residuals plot so that the first Principle Component (PC1) represents the greatest variability or difference between all samples. The second (PC2) represents the regions of difference not already present in PC1. The third (PC3) represents differences not in PC1 and PC2.

 

FAQ ID -2200
Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised?

The supervised mode in the Rotor-Gene ScreenClust HRM Software is designed for data sets with a known number of clusters where each cluster has defined controls. All samples will be grouped into one of the defined clusters.

The unsupervised mode is used if there are no controls for each cluster or if the number of clusters is not known. Based on the data presented, ScreenClust will return the recommended number of clusters and whether to separate the data in 2D or 3D. A user may choose to change either of both of these values.

 

FAQ ID -2202
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits?

Yes, use the assays at a final concentration of 1x with Rotor-Gene Probe Kits on the Rotor-Gene Q cycler.

See trademarks.  

FAQ ID -2126
How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?

Our unique multiplex PCR buffer system with ammonium and potassium ions and Factor MP has been further optimized in QuantiFast and Rotor-Gene Kits. We have also discovered Q-Bond, a buffer component which supports the rapid formation of the polymerase–primer–template complex, leading to reduced annealing times.

FAQ ID -1430
Which qPCR instrument should I use with your RT² qPCR Primer Assays?

Our RT² qPCR Primer Assays may be used on any real-time instrument. qPCR solutions are available for the most popular qPCR instrumentation, including those from QIAGEN, ABI, BioRad, Stratagene.

Instrument-specific protocols are available for selected instruments, and can be accessed at the following link: http://www.sabiosciences.com/pcrarrayprotocolfiles.php

FAQ ID -2714
Investigator Quantiplex: For which real-time cyclers has the kit been validated?
The Investigator Quantiplex Kit has been validated for the Rotor-Gene Q, and the Applied Biosystems 7500 Fast and 7500 Real-Time PCR Systems.
FAQ ID -2567
How is the log graph threshold value (Ct) for the Rotor-Gene Q determined?

Based on the defined standards present, the automatic threshold function of the Rotor-Gene Q scans through all the possible threshold levels until the best fit is determined. This is defined as the R value or correlation coefficient, which is maximized to most closely approach 1.0. If there is no standard present, the threshold can also be set manually.

 

FAQ ID -2088
What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software?

Clusters analyzed in the Rotor-Gene ScreenClust HRM Software are groups of samples with the same melt characteristics. For example, in a single nucleotide polymorphism (SNP) analysis the clusters may represent the genotypes 'wild type', 'mutant' and 'heterozygote'. ScreenClust is designed to group samples into clusters after they are separated based on differences in their melt curves. The clusters can either be defined by control samples (supervised mode) or ScreenClust can determine the appropriate number of clusters (unsupervised mode).

 

FAQ ID -2201
Investigator Quantiplex: Which version of the Rotor Gene-Q software can be used to run the kit?
Using the Investigator Quantiplex on the Rotor-Gene Q: All versions of the Rotor-Gene Q software can be used to run the Investigator Quantiplex.
FAQ ID -2568
Do limiting primer concentrations need to be determined when using the Rotor-Gene Multiplex PCR Kit?

No, that is not necessary. Simply use the primer concentrations specified in the protocols in the handbook supplied with your Rotor-Gene Multiplex PCR Kit.

 

 

FAQ ID -2128
What kind of file is required for hardware-related trouble-shooting?
For hardware related issues, please send the support package to QIAGEN Technical Service. Within the Rotor-gene Q software, click Help and select Send Support Email. In the new window, select the file that relates to the issue and email it to QIAGEN Technical Service.
FAQ ID - 9024
What real-time cycler should I use for my qPCR experiments?
There are several manufacturers of high-quality real-time cyclers. These include QIAGEN's Rotor-Gene Q, Applied Biosystems, BioRad, Stratagene, Eppendorf, Roche, TaKaRa, Fluidigm, and Cepheid. The important thing to keep in mind is that, once you select an instrument to use, you must use compatible Rotor-Gene Discs and tubes, 96 or 384 well plates, and qPCR master mixes that are optimized for use in that particular instrument.  For example, QIAGEN's Rotor-Gene SYBR Green, Probe, and Multiplex real-time master mixes.  
FAQ ID -2670
Is regular calibration needed with the Rotor-gene instrument?
QIAGEN recommends the annual inspection service on Rotor-gene instruments, during which all application-critical modules of the Rotor-gene are inspected and tested and an OTV check is conducted. Performed tests and test results are documented in a GMP/GLP-compliant Report. In addition, the end users can perform the temperature calibration in the lab as needed using the Rotor-Disc OTV kit.

Note: The Rotor-Disc OTV kit requires the Rotor-Disc 72 Rotor and Rotor-Disc 72 locking ring.
FAQ ID -9025
Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q?

Yes. If the reaction efficiency between two PCR runs is not expected to vary, importing a standard curve from a previous run allows to estimate concentrations when a standard curve for the current run is not available. Curves can be imported from another channel, or from another run by clicking on 'Import Curve' in the Rotor-Gene Q software. Standard curves can be adjusted such that only the efficiency of the source curve is imported into the current run. Whether a standard curve should be adjusted depends on the PCR chemistry used. To adjust a standard curve, use a reference with a known concentration in the target run.

 

FAQ ID -2093
Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?

The majority of the Rotor-Gene Kit data shown in our literature has been generated with the help of the QIAgility instrument. We did not observe any problems during the pipetting steps.

 

FAQ ID -2116
Can the Rotor-gene software run on a computer with Windows 7 operating system?

The Rotor-Gene Q software version v2.2 and lower versions require a computer with Windows XP or Vista operating systems. The Rotor-Gene Q software v2.3 or higher versions can run with Windows XP or Windows 7 32-bit and 64-bit operating systems.

Rotor-Gene 6000 software requires a computer with Windows XP or Vista operating system. QIAGEN will no longer support Rotor-Gene 3000 instruments and Rotor-Gene 6.0 software starting from January 1, 2014

FAQ ID -9021
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit?

Yes, simply use the assays at a final concentration of 1x with the Rotor-Gene Multiplex PCR Kit.

See trademarks

FAQ ID -2131
Can Vapor-Lock be used with samples processed on the QIAgility?

Vapor-Lock is fully compatible with the QIAgility instrument for high-precision, automated reaction setup. It is also highly suited for use with the Rotor-Gene Q cycler. For support to program your QIAgility instrument for use with Vapor-Lock, please contact your local QIAGEN Technical Support Department.

 

 

FAQ ID -2153
Can I import a standard curve from a previous PCR run on the Rotor-Gene Q?

Use of endogenous control genes corrects for variation in RNA content, variation in reverse-transcription efficiency, possible RNA degradation or presence of inhibitors in the RNA sample, variation in nucleic acid recover, and differences in sample handling. The endogenous control gene ought to have consistent expression levels between samples and among treatment conditions, and ideally has a similar expression level to that of the genes of interest. Genes commonly used as references can be found at the QuantiTect Primer Assays as endogenous controls.

FAQ ID -9027
Do QuantiTect Primer Assays also work with Rotor-Gene SYBR Green PCR Kits?

We have performed numerous tests comparing the performance of Rotor-Gene SYBR Green PCR Kits and QuantiTect Kits with QuantiTect Primer Assays. Due to the optimized ion concentrations in the PCR buffers, both perform equally well with QuantiTect Primer Assays and do not require any adjustment of primer concentration.

 

FAQ ID -2123
During data analysis, how should the threshold be set in the Yellow channel on the Rotor-Gene Q cycler to analyze the Internal Control from the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit
On the Rotor-Gene Q, setting the threshold in the Yellow channel to an absolute value of 0.05 will give satisfactory results in most cases.
FAQ ID -2607
What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits?

Rotor-Gene Kits are specifically developed for the Rotor-Gene Q PCR Cycler. The unique rotary system of the cycler combined with the kits’ proprietary buffer system enable ultrafast cycling. Rotor-Gene Kits do not contain ROX dye since no normalization to a passive reference is required. Also, Rotor-Gene Kits do not contain dUTP; therefore, UNG pretreatment is not possible.

 

FAQ ID -2119
Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q?

No. The speed at which the samples spin on the Rotor-Gene Q is not high enough to apply any significant centrifugal force on them.

 

 

 

FAQ ID -2081
How fast does the rotor spin during a run on the Rotor-Gene Q?

The sample rotor of the Rotor-Gene Q spins continuously at a speed of 400 rpm during a run.

 

FAQ ID -2080
Can the Rotor-Gene Multiplex RT-PCR Kit be used on other cyclers?

The specific features of Rotor-Gene Kits and Rotor-Gene cyclers work synergetically to enable an ultrafast-cycling protocol. We do not guarantee that the performance of the Rotor-Gene Multiplex RT-PCR Kit with the same cycling protocol will be the same on other cyclers.

 

 

FAQ ID -2142
Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers?

Yes. Rotor-Gene Kits will also work on the Rotor-Gene 6000 and Rotor-Gene 3000 PCR cyclers with the cycling conditions specified in the Rotor-Gene kit handbooks.

 

FAQ ID -2121
Must I fill blank positions with empty tubes when running sample numbers lower than the rotor capacity of the Rotor Gene Q?
Yes, all empty positions in the rotor of the Rotor-Gene Q have to be filled with empty tubes. This guarantees optimal temperature repartition in the Rotor-Gene Q chamber.
FAQ ID -9029
What is the residuals plot in the Rotor-Gene ScreenClust HRM Software?

Once all melt curves are normalized, they are on a comparable basis. To make the information within each curve more useful for comparison, each curve is differentiated. The Residuals Plot is a plot of the difference between each sample and the composite median of all the samples after differentiation. The Residuals Plot of the Rotor-Gene ScreenClust HRM Software is different from a "Difference Plot" known from standard HRM software packages.

 

FAQ ID -2199