QIAamp Virus BioRobot MDx Kit

在BioRobot MDx全自动核酸分离纯化系统上,自动纯化无细胞体液中的病毒DNA和RNA

Products

QIAamp Virus BioRobot MDx Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。
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QIAamp Virus BioRobot MDx Kit (12)

Cat. No. / ID:  965652

For 12 x 96 preps for use on the BioRobot MDx; includes: 12 QIAamp 96 Plates, RNase-free buffers, QIAGEN Protease, Elution Microtubes CL, caps, S-Blocks, carrier RNA
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Features

  • 快速纯化高品质的即用型病毒DNA和RNA
  • 无需有机试剂提取或乙醇沉淀
  • 重复性好,产量高
  • 完全去除污染物和抑制剂

Product Details

QIAamp Virus BioRobot MDx Kit使用经验证的QIAamp硅胶膜技术,在BioRobot MDx全自动核酸分离纯化系统上自动纯化病毒核酸。全程自动化运行无需手动操作,2.5小时内即可完成,其间完成条形码读取和过程存档。

Performance

QIAamp Virus BioRobot MDx对DNA和RNA纯化,回收率和纯度相同,且96孔板样品间未检测到交叉污染。可处理多至265 µl的无细胞体液,标准洗脱体积为75 µl。使用该试剂盒可高度灵敏的检测病毒,经由命中率研究验证(见表)。该系统更深层的优势在于处理过程的高度可靠性和自动化处理的简便性,为纯化病毒核酸提供了高效体系。QIAamp Virus BioRobot MDx为多种下游应用提供可靠结果。

使用QIAamp Virus BioRobot MDx Kit准确率高
PCR 产物(n=96) 仪器 1 仪器 2
平均CT 标准差 %CV 平均 CT 标准差 %CV
模拟病毒 28.26 0.10 0.35 28.38 0.18 0.65
内参 31.98 0.16 0.51 31.87 0.22 0.70
装载的RNA作为模拟RNA病毒分子(每个病毒含25,000个拷贝)加入血浆中。使用QIAamp Virus BioRobot MDx流程在两台仪器上处理相同的96个样本。内参RNA加到裂解缓冲液中。使用TaqMan RT-PCR 体系扩增RNA。计算模拟病毒和内参的平均CT 值、标准差和 %CV。(数据由 QIAGEN R&D部门提供)

Principle

QIAamp Virus BioRobot MDx Kit使用快速的96孔板自动化流程,简化了从无细胞体液中纯化病毒RNA和DNA的流程,无需苯酚-氯仿抽提。核酸特异性结合到QIAamp硅胶膜上,污染物流出。通过两步高效洗涤完全去除二价阳离子和蛋白质等PCR抑制剂,再用水或试剂盒中的缓冲液洗脱结合在离心柱上的核酸。QIAamp技术从无细胞体液中纯化的病毒RNA和DNA,可即时用于PCR和印迹实验。

全程自动化运行无需手动操作,2.5小时内即可处理96个样本(包括20分钟的装载检查),其间完成条形码读取和过程存档。该体系的优势在于纯化流程的高度可靠性和自动化处理的便捷性,为实验室内病毒核酸纯化提供了高效体系。QIAamp样本制备技术得到完全验证认可。

Procedure

试剂盒提供优化的缓冲液高效裂解样本,稳定核酸并促进其选择性结合到QIAamp硅胶膜上。裂解液中添加乙醇并上样到QIAamp 96孔板。使用洗涤缓冲液去除杂质,最后用水或低盐缓冲液洗脱得到即时可用的核酸(参见" Protocol")。
See figures

Applications

QIAamp Virus BioRobot MDx Kit使用经验证的QIAamp硅胶膜技术,在BioRobot MDx全自动核酸分离系统上自动高通量纯化病毒RNA和DNA。96孔板可灵活处理32–96个样本。样本来源包括:

  • 血浆和血清
  • 脑脊液 
  • 其他无细胞体液
  • 细胞悬液

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsPCR, qPCR, real-time PCR
Samples per run (throughput)36-92 samples per run
TechnologySilica technology
Main sample typeSerum, plasma
Elution volume75 µl
ProcessingAutomated
For automated processingBioRobot MDx Workstation
Sample amount265 µl
Format96-well plates
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinViral DNA, viral RNA
Time per run or per prep<2.5 hours
Yield>90% recovery

Resources

产品介绍与指南 (2)
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
试剂盒操作手册 (1)
For simultaneous purification of viral RNA and DNA from plasma, serum, cell-culture supernatants, and cell–free body fluids using the BioRobot MDx
Safety Data Sheets (1)
Certificates of Analysis (1)
Brochures & Guides (2)
Kit Handbooks (1)
For simultaneous purification of viral RNA and DNA from plasma, serum, cell-culture supernatants, and cell–free body fluids using the BioRobot MDx

FAQ

What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

FAQ ID -761
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12