PAXgene Blood miRNA Kit

从全血样品中纯化miRNA和总RNA

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PAXgene Blood miRNA Kit (50)

Cat. No. / ID: 763134

For 50 RNA preps: PAXgene Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, RNase-Free DNase, RNase-Free Reagents and Buffers; to be used in conjunction with PAXgene Blood RNA Tubes

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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

集样本收集、稳定和纯化于一体
高效纯化miRNA和总RNA
在18–25°C条件下RNA稳定3天
-20°C或-70°C条件下至少稳定50个月

Product Details

PAXgene Blood miRNA System包含PAXgene Blood miRNA Kit，用于纯化大于18 nt的总RNA（含miRNA）；以及用于血液收集、稳定和运输的PAXgene Blood RNA Tubes（BD提供，货号762165）。该试剂盒基于硅胶膜技术，以离心柱方式纯化RNA。纯化可使用小离心机手工完成，或者在QIAcube全自动核酸纯化仪上自动进行。配合PAXgene Blood RNA Tubes，系统提供了一个样本分析前处理的标准方案，包括从收集、稳定到RNA纯化的全过程（包括miRNA），适用于分子分析。

Performance

血液样本在PAXgene Blood RNA Tubes中收集后可安全保存，或转移到15–25°C下保存72小时，在2–8°C下保存5天，在–20°C或–70°C下保存至少50个月，而不会发生明显的RNA降解或转录水平的变化。使用PAXgene Blood miRNA Kit从稳定的血液样本中纯化大于18个核苷酸的总RNA，包括mRNA和miRNA等小RNAs（参见""）。使用QIAGEN miScript PCR System（参见""）或其他real-time RT-PCR系统可分析miRNA种类。

See figures

High yields of small RNA species.

Analysis of miRNA species.

Principle

血液样本收集在PAXgene Blood RNA Tubes中。管中含有的试剂可裂解血液细胞并立即稳定胞内RNA，保护基因表达谱。RNA稳定对于可靠的下游基因表达分析非常重要。收集血液后如果没有立即稳定，就会造成RNA降解、转录本的上调或下调。

Procedure

使用PAXgene Blood miRNA Kit，运用经验证的PAXgene硅胶膜技术可从稳定的血液样本中纯化大于18个核苷酸的RNA（包括miRNA）。可手工操作完成纯化或在QIAcube全自动核酸纯化仪上自动进行（参见"The QIAcube"）。

See figures

Applications

纯化的总RNA可直接使用，适合多种下游应用，包括：

RT-PCR和real-time RT-PCR
差异显示
cDNA合成
Northern印记、点印记和狭缝印记
引物延伸
Poly A⁺ RNA筛选
RNase/S1核酸酶保护分析
芯片分析

Supporting data and figures

Analysis of miRNA species.

Blood was collected in PAXgene Blood RNA Tubes and stored for 20–24 hours at room temperature before freezing at –15 to–30°C. RNA was purified using the PAXgene Blood RNA Kit or the PAXgene Blood miRNA Kit, as indicated. miRNA species were analyzed using the QIAGEN miScript PCR System, with the indicated miScript Primer Assays. The lower C_T values for RNA purified using the PAXgene Blood miRNA Kit indicate higher amounts of those miRNA species.

Analysis of miRNA using miRCURY LNA miRNA Probe PCR Assays with miRCURY LNA miRNA Probe PCR System

Specifications

Features	Specifications
Applications	PCR, real-time PCR, qPCR, NASBA, cDNA synthesis, miRNA Assays
Elution volume	40 µl
Time per run or per prep	90 minutes
Technology	Silica technology
Main sample type	Whole blood
Yield	>3 µg (total RNA)
Format	Spin column
Processing	Manual (centrifugation) or on QIAcube
Sample amount	2.5 ml

Resources

For collection, transport, and storage of whole blood and stabilization and purification of intracellular RNA

For purification of intracellular RNA, including miRNA, from whole blood

Download Safety Data Sheets for QIAGEN product components.

Voss et al., AMP 2009

Voss et al., qPCR 2009

Kruhoffer et al., AMP 2007

This note is to inform you that the street address for PreAnalytiX GmbH has changed from “Feldbachstrasse” to “Garstligweg 8”. Please be informed that the update of the product labeling to the new address is ongoing.

In order to reduce paper consumption and oblige the growing number of customers requesting an environmentally friendly alternative to traditionally printed handbooks, we are now providing kit handbooks for Research Use Only (RUO) PreAnalytiX kits on our website only.

Comparison of miRNA recovery from the PAXgene Blood miRNA System and the Tempus Blood RNA Tube processed with the Tempus Spin RNA Isolation Kit

Typical total RNA yields from PAXgene Blood RNA Tubes processed with the PAXgene Blood miRNA Kit

For purification of miRNA and total RNA from whole blood.

FAQ

You can find additional information relating to the PreAnalytiX PAXgene products on the PreAnalytiX website .

FAQ ID - 3515

No. The PAXgene Blood miRNA Kit is ‘For Research Use Only’. Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

FAQ ID -2494

Typical yields of RNA isolated from 2.5 ml healthy, human, whole blood (4.8 x 10⁶ –1.1 x 10⁷ leukocytes/ml) are ≥3 μg for >95% of the samples processed (Refer to Technical Note) . Since yields are highly donor-dependent, individual yields may vary.

FAQ ID - 3481

No. The PAXgene Blood RNA System has been optimized for cellular RNA only. PreAnalytiX offers a dedicated system for collection and isolation of genomic DNA from human whole blood (see PAXgene Blood DNA System) .

FAQ ID - 3483

For the isolation of microRNA (miRNA) specifically, we have developed the miRNeasy Mini Kit and the miRNeasy 96 Kit for isolation from cells and tissues. We also have the PAXgene Blood miRNA and Tissue miRNA kits for isolation from blood stored in PAXgene Blood RNA tubes and PAXgene Tissue Containers, respectively. Other miRNA isolation supplementary protocols can also be found by searching our comprehensive protocols at http://www.qiagen.com/literature/default.aspx?WT.svl=m.

FAQ ID -115

Up to 12 samples can be processed in a single run.

FAQ ID - 3482

If the PAXgene Blood miRNA Kit is used, an enrichment of small RNAs as part of the total RNA is achieved. The enrichment may vary between different miRNA species and samples, but on average an enrichment of about three or more CT-values can be expected.

FAQ ID -2495

Typically, the amount of genomic DNA (gDNA) in eluates of the PAXgene Blood miRNA Kit is very low as long as the kit is stored and used according to the instructions of the PAXgene Blood miRNA Kit Handbook. For downstream applications that are especially sensitive to gDNA contamination, a specialized protocol for the QIAcube helps reduce gDNA content further and can be downloaded from the QIAcube product page.

FAQ ID - 3637

2.5 ml whole human blood collected in PAXgene Blood RNA Tubes

FAQ ID - 3478

Stability data are not yet available, but a stability study was started.

FAQ ID - 3480

The kit can be used for 50 RNA isolations.

FAQ ID - 3477

Yes. Samples have to be incubated at least 2 h at room temperature (18-22°C). Incubation of the PAXgene Blood RNA Tube overnight may increase yields slightly in some cases. Room temperature storage for up to three days has no effect on the RNA profile. During the preparation of the RNA the manual pretreatment consist of a centrifugation and a pellet resuspenion step (see the PAXgene Blood miRNA Handbook ).

FAQ ID - 3479