TissueLyser LT

用于低到中通量样本破碎,适用于分子分析

S_1563_GEF_TLLT_50

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TissueLyser LT

Cat. No. / ID:  85600

Compact bead mill, 100–240 V AC, 50–60 Hz; requires the TissueLyser LT Adapter, 12-Tube (available separately)
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TissueLyser LT
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TissueLyser LT 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 同时破碎至多12个样本
  • 仪器结构紧凑
  • 配合可冷却的适配器,防止生物分子降解
  • 可处理各种类型的样本,获得高重复性结果
  • 与很多实验室流程兼容

Product Details

TissueLyser LT是一款小型球磨仪,可同时快速、高效的破碎至多12个样品。该通量与自动化样品制备工作站QIAcube全自动核酸纯化仪一致,可配合值得信赖的QIAGEN离心柱试剂盒使用。用不锈钢珠或玻璃珠高速震荡放置于2 ml微型离心管中的样品,实现同步破碎和匀浆。TissueLyser LT必须配合可冷却的适配器TissueLyser LT Adapter使用,该适配器在破碎过程中可固定试管。其他附件包括研磨珠和研磨珠分配器。

Performance

TissueLyser LT可高效破碎人类、动物和植物组织、细菌和酵母,确保后续DNA、RNA和蛋白质纯化中产量重复性好(参见" Effective tissue disruption")。心脏和大脑等难裂解组织,也可使用TissueLyser LT处理。破碎之后DNA、RNA和蛋白质保持完整(参见" Pure RNA with high RIN values"、" Intact protein suitable for all types of analysis"、" High-quality DNA from animal tissues"、" High-quality DNA from plant tissues"和" Intact RNA from plant tissues"),确保下游应用中可靠的分析。

TissueLyser LT的破碎效果与TissueLyser II相当(参见" Effective tissue disruption"、" Intact protein suitable for all types of analysis"、" High-quality DNA from animal tissues"和" High-quality DNA from plant tissues")。

See figures

Principle

基因分型、基因表达分析和蛋白质组学研究均要求对生物样本进行高效破碎,以确保获得高产的DNA、RNA和蛋白质。TissueLyser LT可快速、彻底的破碎样本,彻底释放生物分子,并可同时对样本进行匀质化,协助后续使用的QIAGEN试剂盒进行纯化(参见下表)。

QIAGEN提供从样本收集到DNA、RNA和蛋白质纯化的完整解决方案,TissueLyser LT是其中关键的组成部分。TissueLyser LT适用于QIAGEN手动样本制备试剂盒(参见下表),也是QIAGEN中通量样本制备和分析自动化解决方案的补充(参见下表)。包括应用QIAGEN离心柱试剂盒自动纯化样品的QIAcube全自动核酸纯化仪,以及对DNA和RNA进行毛细管凝胶电泳的QIAxcel。

QIAGEN纯化试剂盒配合QIAGEN破碎体系
纯化的分析物 样本类型 QIAGEN试剂盒
RNA 容易裂解的组织 RNeasy Kits
RNeasy Plus Kits
RNeasy Protect Kits
RNA 富含纤维的组织 RNeasy Fibrous Tissue Kits
RNA 各种类型的组织 RNeasy Lipid Tissue Kits
RNeasy Universal Tissue Kits
RNA 植物组织 RNeasy Plant Mini Kit
RNA 酵母 RNeasy Kits
RNA 细菌 RNeasy Protect Bacteria Kits
microRNA 各种类型的组织 miRNeasy Kits
DNA 人类组织 QIAamp DNA Kits
DNA 动物组织 DNeasy Blood & Tissue Kits
蛋白质 组织 Qproteome Mammalian Protein Prep Kit
磷蛋白 组织 PhosphoProtein Purification Kit
糖蛋白 组织 Qproteome Glycoprotein Kits
DNA和RNA 组织 AllPrep DNA/RNA Kits
DNA、RNA和蛋白质 组织 AllPrep DNA/RNA/Protein Mini Kit
QIAGEN medium-throughput automation
仪器 目的 通量
QIAcube 纯化DNA、RNA和蛋白质 每批多达12个样本
EZ1 Advanced 从人类样本中纯化DNA和RNA 每批多达6个样本
EZ1 Advanced XL 从人类样本中纯化DNA和RNA 每批多达14个样本
QIAxcel 对DNA片段和RNA进行电泳分析 每批多达96个样本
QIAgility 反应体系构建 每批多达384个样本
Rotor-Gene Q real-time PCR和HRM分析 每批多达100个样本
PyroMark Q24 甲基化和突变分析 每批多达24个样本

Procedure

TissueLyser LT与TissueLyser LT Adapter配合使用。每次操作可将多达12个2 ml的试管放置于适配器上,每管含一个样本和一颗研磨珠。适配器牢固的固定在TissueLyser LT的活塞上。活塞快速上下移动,使得研磨珠震动、研磨,可同时破碎并匀质化样本。

TissueLyser LT的大小只有15 cm x 27 cm,可放置于任何实验室。由于每个样本都安全的密封在试管内,可用TissueLyser LT同时纯化多个样本,而无交叉污染的风险。破碎新鲜或冷冻样品,可用干冰预先冷却TissueLyser LT Adapter,避免核酸和蛋白质降解(参见" High-quality DNA from plant tissues"和" Intact RNA from plant tissues")。组织可储存于 Allprotect Tissue Reagent(稳定DNA、RNA和蛋白质)或储存于RNAlater RNA Stabilization Reagent(稳定RNA),无需预先冷却适配器。

See figures

Applications

TissueLyser LT每批可处理多达12个样本,因此是遗传学、转录组学和蛋白质组学研究中获得生物信息的理想解决方案。TissueLyser LT可快速、统一的破碎动物和人类组织、植物组织、细菌和真菌,需与TissueLyser LT Adapter配合使用,TissueLyser LT Adapter可固定12个2 ml的样本管。样本纯化可以手动进行,也可以在QIAcube全自动核酸纯化仪、QIAsymphony SP核酸提取纯化分析仪、QIAxtractor、EZ1 Advanced、EZ1 Advanced XL、BioRobot或BioSprint工作站上自动进行。

Supporting data and figures

Specifications

FeaturesSpecifications
DimensionsWeight: Approx. 7 kg, Width: 150 mm, Depth: 270 mm, Height: 280 mm, The power supply of the TissueLyser LT is compatible with voltages of 100–240 V
Disruption principleHigh-speed shaking of samples in 2 ml microcentrifuge tubes with stainless steel or glass beads
Protocol/main application on this intrumentSample preparation/sample disruption
FeaturesSimultaneous disruption of up to 12 samples. Compact instrument with small footprint. Coolable adapter to prevent biomolecule degradation. Reproducible results with all sample types. Compatible with all laboratory workflows.
TechnologyBead Mill
Kits compatible with instrumentAll kits for purification of RNA/DNA/protein
Typical run time40 sec - 5 min, depending on protocol

Resources

试剂盒操作手册 (3)
For disruption of up to 12 biological samples
For disruption of up to 12 samples on the TissueLyser LT
Safety Data Sheets (1)
Certificates of Analysis (1)
Kit Handbooks (3)
For disruption of up to 12 samples on the TissueLyser LT
For disruption of up to 12 biological samples

FAQ

Are the stainless steel beads magnetic?

Stainless steel beads sold by QIAGEN are not guaranteed to be magnetic. However, you may find some lots of the beads to be magnetic, due to small variations in the chemical compositions (which do not affect the disruptive functions of the beads).

FAQ ID -9180
Can the sample tubes and the TissueLyser LT Adapter be cooled with liquid nitrogen?

No, cooling with liquid nitrogen would make the sample tubes brittle, resulting in breakage of the tubes during the disruption process. Cooling the adapter and sample tubes on dry ice or in a freezer to approx. -80°C is sufficient to prevent the samples from thawing during the disruption process.

 

See:  TissueLyser Virtual Demo for a demonstration of cooling the samples on dry ice before using the TissueLyser LT.

FAQ ID -2549
How long does the sample disruption with the TissueLyser LT take?
Depending on the downstream application and the tissue, the disruption time typically takes 40 sec to 5 min. For more information about the different applications please refer to the TissueLyser LT Handbook.
FAQ ID -2550
Are there any accessories required for sample disruption with the TissueLyser LT?

Yes, disruption and homogenization are achieved through the beating and grinding effect of beads on the sample material as they are shaken together in 2 mL sample tubes. Sample tubes and different beads can be purchased at QIAGEN (see ordering information in the TissueLyser LT Handbook).

 

See:  TissueLyser Virtual Demo for a virtual demonstration of this process.

FAQ ID -2551
Is an O-ring available to seal the lid of the TissueLyser LT Adapter to prevent aerosolization or escape of pathogens during lysis? Can I operate the TissueLyser LT in a laminar air flow hood?

The TissueLyser LT Adapter (69980) is not guaranteed to be airtight and we do not have an O-ring to seal the lid of the adapter.

If you are working with infectious agents, you will need to validate the disruption parameters on your own to make sure the pathogens do not escape outside.

You should find it possible to operate the TissueLyser LT in a laminar air flow hood.


FAQ ID -9116
Do you sell ceramic beads or glass beads for use with TissueLyser II or TissueLyser LT?

We do not sell ceramic beads or glass beads for use with our TissueLyser II or TissueLyser LT instruments. However, we still sell stainless steal beads and tungsten carbide beads. For more information, please go to this link.

FAQ ID -9189
What is the temperature tolerance of TissueLyser LT Adapter? Can I precool the adapter in liquid nitrogen?

TissueLyser LT Adapter is tolerant to temperatures between –78.5°C and +150°C.

Do not freeze the TissueLyser LT Adapter or the sample tubes in liquid nitrogen. The adapter and tubes may break and may cause injury.

However, the insert of the TissueLyser LT Adapter, the sample tubes, and grinding beads can be placed on dry ice for cooling prior to disruption.


FAQ ID -9111
What are the dimensions of the TissueLyser LT?

Height: 280 mm

Width: 150 mm

Depth: 270 mm


FAQ ID -9114
How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit?

Efficient DNA isolation requires thorough sample disruption and digestion.

Although the QIAamp and DNeasy procedures requires no mechanical disruption of the tissue sample, the lysis time will be reduced if the sample is ground in liquid nitrogen or mechanically homogenized in advance. For mechanical homogenization, a rotor–stator homogenizer, such as the QIAGEN TissueRuptor, or a bead mill, such as the QIAGEN TissueLyser, can be used.

To improve digestion of tough tissue samples, Proteinase K incubation at 56°C can be performed overnight. DNA yields may be improved by increasing the amount of Proteinase K or by adding additional proteinase K after several hours of digestion.  

FAQ ID -374
Can I use 3 mm beads with a 5 mm or 7 mm bead dispenser?
Unfortunately, this is not possible. The 3 mm beads are not compatible with the 5 mm or 7 mm TissueLyser Single Bead Dispenser, as this could result in dispensation of multiple beads, and/or the dispenser may get blocked.
FAQ ID -9185
The disruption of my sample worked well with the TissueLyser LT, but I am not satisfied with the RNA yield. What do you suggest?
The RNA yield can be improved by choosing a specialized RNeasy Kit, which is optimally adapted to the starting material (e.g., RNeasy Lipid Tissue Kits for brain tissue or RNeasy Fibrous Tissue Kit for heart).
FAQ ID -2555
My TissueLyser LT is out of warranty and does not work anymore. Do you offer repair?

We are sorry, but we do not offer repair on the TissueLyser LT.


FAQ ID -9112
Can I use Buffer ATL for disruption of my samples with TissueRuptor, TissueLyser II, or TissueLyser LT instruments?

Buffer ATL can produce foam when used in conjunction with the TissueRuptor. You can add 0.5% final volume of Reagent DX (cat. no. 19088) to prevent the formation of foam.

FAQ ID -9143
What sample numbers can be processed on the TissueLyser LT?

Any number of samples between 1 and 12 can be processed on the TissueLyser LT. If processing either 1 sample or 11 samples, load an additional, empty sample tube to balance the TissueLyser LT Adapter. For more information please see the TissueLyser LT Handbook.

 

See: TissueLyser Virtual Demo for a virtual demonstration on how the TissueLyser LT works.

FAQ ID -2547
What tubes do you recommend for the TissueLyser LT Adapter?

We recommend using 2 ml Sample Tubes RB (cat. no. 990381) with our TissueLyser LT.


FAQ ID -9113
Are the beads RNase free or sterile?

We do not guarantee the stainless steel beads to be RNase free. However, when used according to our specifications for disruption of biological samples, no special treatment is needed to make the beads RNase free. This is because our chaotropic lysis buffers will usually inactivate these and RNases contained in the samples themselves.

FAQ ID -9181
Are the stainless beads ready to use? Do I need to pretreat them with acid before use?

Our stainless steel beads and tungsten carbide beads are ready to use.

FAQ ID -9183
What are the dimensions of the TissueLyser LT?

The TissueLyser LT has the following weight and dimensions:

  • Weight: Approx. 7.35 kg (net); approx. 9 kg (gross)
  • Width: 150 mm
  • Depth: 270 mm
  • Height: 280 mm
FAQ ID -2545
I am working with very fibrous and difficult tissues such as cartilage, heart, muscle and skin. Do you have any recommendations for disruption with TissueLyser LT?

When disrupting tough or very tough samples, we recommend using one and two 7 mm stainless steel beads, respectively, instead of one 5 mm stainless steel bead, to guarantee optimal disruption.

Do not exceed the recommended amount of tissue per sample tube. Please refer to the TissueLyser LT Handbook for our recommendations of starting sample amount for purification of DNA, RNA, or proteins from various sample types.

You may need to increase the time and/or intensity of homogenization from what is recommended in the TissueLyser LT Handbook. But please be aware that this may lead to some fragmentation of gDNA.


FAQ ID -9115
What is the typical amount of tissue used for disruption with the TissueLyser LT?
The amount of starting material is dependent on the sample material that should be disrupted. For plant tissues up to 100 mg, and for human or animal tissues 25 to 30 mg, can be disrupted with the TissueLyser LT. When disrupting yeast up to 5 x 10^7 cells, and when disrupting bacteria up to 7.5 x 10^8 cells, can be used. Using more than the recommended amount of starting material will significantly decrease disruption efficiency.
FAQ ID -2552
Can the samples on the TissueLyser LT be protected from degradation of nucleic acids or proteins?

Yes, if using fresh samples that are not stabilized with a stabilization reagent (e.g., RNAprotect Tissue Reagent or Allprotect Tissue Reagent) the TissueLyser LT Adapter can be precooled on dry ice. During the disruption process the adapter will passively cool the samples to prevent heating and degradation of nucleic acids or proteins.

See:  TissueLyser Virtual Demo for a virtual demonstration of this precooling process with the TissueLyser LT.


FAQ ID -2548
Can the TissueLyser LT Adapter be removed for cleaning?

Yes, the TissueLyser LT Adapter can completely be removed from the instrument for cleaning. For more information on how to clean or decontaminate the instrument and the adapter please refer to chapter 6.1 in the TissueLyser LT User Manual on regular maintenance. The TissueLyser LT Adapter must not be autoclaved.

 

See:  TissueLyser Virtual Demo for a virtual demonstration on how the TissueLyser LT Adapter is used 

FAQ ID -2546
Can I use the TissueLyser LT for disrupting bone? Is this instrument powerful enough?

The largest and most efficient beads that can be used with the TissueLyser LT are the 7 mm stainless steel beads. We suggest pre-disrupting teeth or bone into smaller pieces (e.g., using a hammer) and then use the resulting pieces for further disruption in the TissueLyser LT. In this case, we suggest cooling the samples and the adapter on dry ice prior to disruption, rather than disruption in a lysis buffer.

Please note that the adaptor and tubes should not be cooled using liquid nitrogen, as this could lead to breakage of the TissueLyser Adapter and the sample tubes and may cause injury.


FAQ ID -9110
Can I use the 2 ml Sample Tubes RB (cat. no. 990381) with TissueLyser II and TissueLyser LT?

Yes, the 2 ml Sample Tubes RB (cat. no. 990381) can be used with both TissueLyser II and TissueLyser LT.

FAQ ID -9188
What kind of sample tubes are recommended to use with the TissueLyser LT?
The TissueLyser LT has been thoroughly tested with the Sample Tubes RB (2 ml) catalog number 990381. We do not recommend the use of any other tubes with the instrument.
FAQ ID -2553
How to clean the stainless steel beads?

Stainless steel beads can be reused for DNA extractions with DNeasy Plant Kits. Used beads can be recovered from cell-debris and cleaned using the procedure below:

  1. Close the cap of the 2 ml microtube and briefly vortex to dislodge the bead and pellet from the bottom of the tube. If using grinding jars for the disruption of large sample volumes, skip to step 2.
  2. Empty the contents of the tubes/jars into a sieve and rinse the beads thoroughly with water.
  3. Incubate beads in 0.4 M HCl for 1 min at room temperature (15–25°C) to degrade any DNA and avoid cross-contamination in future preparations.
  4. Rinse beads thoroughly with distilled water to remove the HCl.
  5. Dry beads before use.

You can also find these instructions in Appendix B of the DNeasy Plant Handbook.

FAQ ID -9179
What size bead should I use for tougher samples with the TissueLyser LT?

In general, disruption works very well using one stainless steel bead with a diameter of 5 mm. However, for tougher samples we recommend the use of two 7mm stainless steel beads to optimize the effectiveness of disruption.

 

See:  TissueLyser Virtual Demo for a demonstration on how these beads are used with the TissueLyser LT.

FAQ ID -2554
Do you sell the 3 mm stainless steel beads?

We do not sell 3 mm stainless steel beads for use with our TissueLyser instruments.

FAQ ID -9187