QIAamp DNA FFPE Tissue Kit

用于从福尔马林固定石蜡包埋组织中纯化基因组 DNA

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

QIAamp DNA FFPE Tissue Kit (50)

Cat. No. / ID: 56404

用于 50 次 DNA 制备：50 QIAamp MinElute Columns、Proteinase K、Buffer、Collection Tubes (2 ml) 使用我们的新一代 QIAamp DNA FFPE Advanced Kit 来提高性能。

Copy order details

QIAamp DNA FFPE Tissue Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

快速纯化即用型高品质 DNA
稳定的高产量
完全去除污染物和抑制物

Product Details

QIAamp DNA FFPE Tissue Kit 专为从福尔马林固定石蜡包埋组织切片中纯化 DNA 而设计。该试剂盒使用特殊的裂解条件来释放组织切片中的 DNA 并克服福尔马林交联核酸造成的抑制作用。该试剂盒使用 QIAamp MinElute Spin Columns 来纯化小体积中的高品质 DNA。使用 QIAamp DNA FFPE Tissue Kit 进行的 DNA 纯化可在 QIAcube Connect 上自动完成。

试用下一代 QIAamp DNA FFPE Advanced Kit，高效回收 DNA 并选择性地去除伪像。

Performance

经 QIAamp DNA FFPE Tissue Kit 纯化的 DNA 可用于多种下游应用，如单核苷酸多态性（Single Nucleotide Polymorphism，SNP）和短串联重复序列（Short-Tandem Repeat，STR）基因分型以及药物基因组学研究。用 QIAamp DNA FFPE Tissue Kit 纯化的 DNA 也可用于 PCR（见图“”）。

See figures

对从 FFPE 组织样本中纯化的 DNA 进行 PCR 分析。

Principle

QIAamp DNA FFPE Tissue Kit 使用成熟的 QIAamp MinElute 技术纯化小体积或小尺寸样本中的基因组和线粒体 DNA。该试剂盒将硅胶膜的选择性附着属性与 20-100 µl 的灵活洗脱体积相结合。

裂解条件经过专门优化，可从 FFPE 组织切片中高效纯化基因组 DNA 而不需要进行过夜孵育。蛋白酶 K 降解后，升温孵育可以部分释放被福尔马林交联的 DNA，从而可以提高产量，以及改善 DNA 在下游检测中的性能。注意，从 FFPE 样本中分离出的 DNA 通常在分子量上低于新鲜或冷冻样本中的 DNA。样本破碎化的程度取决于样本的类型、制作时间及其固定条件。

Procedure

QIAamp DNA FFPE Tissue 操作流程包括 6 个步骤：脱蜡、裂解、加热、结合、洗涤和洗脱（见流程图“ 操作流程”）。样本裂解后的 QIAamp DNA FFPE Tissue 操作流程很简单，非常适合用于同时处理多个样本，不到 30 分钟就能得到纯净的 DNA。

See figures

QlAamp DNA FFPE Tissue Kit 操作流程。

Applications

QIAamp DNA FFPE Tissue Kit 专为从福尔马林固定石蜡包埋组织中纯化 DNA 而设计。

Supporting data and figures

对从 FFPE 组织样本中纯化的 DNA 进行 PCR 分析。

使用 QIAamp DNA FFPE Tissue Kit 从不同的 FFPE 组织样本中纯化基因组 DNA。通过定量 real-time PCR 扩增 prnp 基因的产物（78 bp、464 bp 和 727 bp）。

Specifications

Features	Specifications
Applications	Real-time PCR、STR 分析、LMD-PCR
Processing	手动
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	基因组 DNA、线粒体 DNA
Elution volume	20-100 µl
Format	离心柱
Technology	硅胶膜纯化技术
Main sample type	福尔马林固定石蜡包埋组织样本
Sample amount	最多 8 个切片，每个厚度最大 10 µm，表面积最大 250 mm2

Resources

Sample to Insight solutions for successful molecular analysis

High-quality, nucleic acid purification for successful PCR and NGS experiments.

Second edition — innovative tools

Critical factors for molecular analysis of FFPE samples

Download Safety Data Sheets for QIAGEN product components.

High-quality, nucleic acid purification for successful PCR and NGS experiments.

Second edition — innovative tools

Sample to Insight solutions for successful molecular analysis

Critical factors for molecular analysis of FFPE samples

Publications

Gender-related survival differences associated with EGFR polymorphisms in metastatic colon cancer.

Press OA; Zhang W; Gordon MA; Yang D; Lurje G; Iqbal S; El-Khoueiry A; Lenz HJ;

Cancer Res; 2008; 68 (8):3037-42 2008 Apr 15 PMID:18413774

Discrepancy between CYP2D6 phenotype and genotype derived from post-mortem dextromethorphan blood level.

Bailey B; Daneman R; Daneman N; Mayer JM; Koren G;

Forensic Sci Int; 2000; 110 (1):61-70 2000 May 8 PMID:10802201

FAQ

The MinElute spin columns included in the following kits should be stored at 2–8°C upon arrival: AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro.

Short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect the performance. However, for optimal performance and quality, storage temperature should not exceed 25°C.

FAQ ID - 3560

The composition of Buffer ATE is:

- 10 mM Tris-Cl pH 8.3

- 0.1 mM EDTA

- 0.04% NaN3 (sodium-azide)

FAQ ID -3122

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728