QIAamp 96 DNA Swab BioRobot Kit

用于自动化高通量拭子 DNA 纯化

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QIAamp 96 DNA Swab BioRobot Kit (12)

Cat. No. / ID:  965842

用于 12 次 96 DNA 制备:12 个 QIAamp 96 孔板、缓冲液、QIAGEN Proteinase K、AirPore 多孔薄膜片、胶带纸本、S-Blocks、带采样微量管 (1.2 ml) 的试管架、盖子
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QIAamp 96 DNA Swab BioRobot Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 快速纯化高质量 DNA
  • 无需有机萃取或酒精沉淀
  • 稳定的高产量
  • 去除污染物和抑制剂

Product Details

QIAamp 96 DNA Swab BioRobot Kit 采用成熟可靠的 QIAamp 硅膜技术,可在 BioRobot Universal System 上从多达 192 个拭子中自动纯化 DNA。最多可处理 192 份样本,处理时间不到 2.5 小时。该程序经过优化,适用于带有塑料轴和棉花或 DACRON 头的风干拭子,但也可使用其他类型的拭子。

Performance

成熟可靠的 QIAamp 96 技术可确保孔间 DNA 回收率和纯度的一致性,且样本之间无交叉污染(见“ 交叉污染测试”图)。酶抑制剂的有效去除确保了 TaqMan®、LightCycler 和 iCycler 分析等下游应用的可靠性能(见“ 性能可靠”图)。

使用 QIAamp 96 DNA Swab BioRobot Kit 分离的 DNA 可广泛应用于分子诊断和法医科学的多种领域:

See figures

Principle

无需酚-氯仿提取。DNA 与 QIAamp 硅胶膜特异性结合,而污染物则会穿过膜。PCR 抑制剂(如二价阳离子和蛋白质)会通过四个高效的清洗步骤被完全去除,留下纯净的 DNA,然后在水或试剂盒附带的缓冲液中进行洗脱。QIAamp 技术能从拭子中提取 DNA,以便用于 PCR 和印迹程序。

Procedure

QIAamp 96 DNA Swab BioRobot Kit 采用快速自动化的 96 孔板程序简化了从拭子中分离 DNA 的过程(见流程“ QIAamp 96 DNA Swab BioRobot 程序”图)。该程序经过优化,适用于带有塑料轴和棉花或 DACRON 头的风干拭子,但也可使用其他类型的拭子。QIAamp 96 DNA Swab BioRobot Kit 的典型产量为每支拭子 1–2 µg(口腔拭子),洗脱体积为 150 µl。

QIAamp 样本制备技术已获得完全授权。

See figures

Applications

QIAamp 96 DNA Swab BioRobot Kit 以方便的 96 孔格式提供 QIAamp 技术来满足高通量纯化需求。最多可在 2 小时内纯化 96 个拭子样本的 DNA。样本类型包括:

  • 口腔拭子
  • 咽拭子
  • 眼拭子

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsPCR、印迹
Yield每个拭子 1–2 µg
Format96 孔板
For automated processingBioRobot Universal System、BioRobot Genotyping、BioRobot 9604
Sample amount每孔 1 个拭子
Processing自动
Elution volume150 µl
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein基因组 DNA
Main sample type拭子
Technology硅膜技术
Time per run or per prep<2 小时

Resources

试剂盒操作手册 (1)
For automated purification of genomic DNA from buccal swabs using the BioRobot Universal System, BioRobot Genotyping, or BioRobot 9604
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)
Kit Handbooks (1)
For automated purification of genomic DNA from buccal swabs using the BioRobot Universal System, BioRobot Genotyping, or BioRobot 9604

Publications

Estrogen sulfation genes, hormone replacement therapy, and endometrial cancer risk.
Rebbeck TR; Troxel AB; Wang Y; Walker AH; Panossian S; Gallagher S; Shatalova EG; Blanchard R; Bunin G; DeMichele A; Rubin SC; Baumgarten M; Berlin M; Schinnar R; Berlin JA; Strom BL;
J Natl Cancer Inst; 2006; 98 (18):1311-20 2006 Sep 20 PMID:16985250
Isolation of genomic DNA from buccal swabs for forensic analysis, using fully automated silica-membrane purification technology.
Hanselle T; Otte M; Schnibbe T; Smythe E; Krieg-Schneider F;
Leg Med (Tokyo); 2003; 5 Suppl 1 :S145-9 2003 Mar PMID:12935575

FAQ

What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

FAQ ID -761
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12