QIAamp 96 DNA Swab BioRobot Kit

用于自动化高通量拭子 DNA 纯化

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

QIAamp 96 DNA Swab BioRobot Kit (12)

Cat. No. / ID: 965842

用于 12 次 96 DNA 制备：12 个 QIAamp 96 孔板、缓冲液、QIAGEN Proteinase K、AirPore 多孔薄膜片、胶带纸本、S-Blocks、带采样微量管 (1.2 ml) 的试管架、盖子

登录要查看您的账户定价。

该产品将于 September 30, 2025 起停产，或存货耗尽时停售。

见商标。

QIAamp 96 DNA Swab BioRobot Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

快速纯化高质量 DNA
无需有机萃取或酒精沉淀
稳定的高产量
去除污染物和抑制剂

产品详情

QIAamp 96 DNA Swab BioRobot Kit 采用成熟可靠的 QIAamp 硅膜技术，可在 BioRobot Universal System 上从多达 192 个拭子中自动纯化 DNA。最多可处理 192 份样本，处理时间不到 2.5 小时。该程序经过优化，适用于带有塑料轴和棉花或 DACRON 头的风干拭子，但也可使用其他类型的拭子。

绩效

成熟可靠的 QIAamp 96 技术可确保孔间 DNA 回收率和纯度的一致性，且样本之间无交叉污染（见“ 交叉污染测试”图）。酶抑制剂的有效去除确保了 TaqMan®、LightCycler 和 iCycler 分析等下游应用的可靠性能（见“ 性能可靠”图）。

使用 QIAamp 96 DNA Swab BioRobot Kit 分离的 DNA 可广泛应用于分子诊断和法医科学的多种领域：

遗传疾病测试，如血色沉着病或莱登第五因子
身份鉴定，如 HLA 分型
亲子鉴定（见“ 多重 STR 分析”图和“ GeneScan 分析”图）

查看图表

原理

无需酚-氯仿提取。DNA 与 QIAamp 硅胶膜特异性结合，而污染物则会穿过膜。PCR 抑制剂（如二价阳离子和蛋白质）会通过四个高效的清洗步骤被完全去除，留下纯净的 DNA，然后在水或试剂盒附带的缓冲液中进行洗脱。QIAamp 技术能从拭子中提取 DNA，以便用于 PCR 和印迹程序。

程序

QIAamp 96 DNA Swab BioRobot Kit 采用快速自动化的 96 孔板程序简化了从拭子中分离 DNA 的过程（见流程“ QIAamp 96 DNA Swab BioRobot 程序”图）。该程序经过优化，适用于带有塑料轴和棉花或 DACRON 头的风干拭子，但也可使用其他类型的拭子。QIAamp 96 DNA Swab BioRobot Kit 的典型产量为每支拭子 1–2 µg（口腔拭子），洗脱体积为 150 µl。

QIAamp 样本制备技术已获得完全授权。

查看图表

QIAamp 96 DNA Swab BioRobot 程序。

应用

QIAamp 96 DNA Swab BioRobot Kit 以方便的 96 孔格式提供 QIAamp 技术来满足高通量纯化需求。最多可在 2 小时内纯化 96 个拭子样本的 DNA。样本类型包括：

口腔拭子
咽拭子
眼拭子

辅助数据和图表

使用 QIAamp MinElute Virus Kit 进行 PCR 和 RT-PCR 具有高灵敏度。

图中显示了低病毒滴度时阳性样本百分比。使用 QIAamp MinElute Spin 或 Vacuum Kit 从血浆中纯化了 DNA 病毒核酸，然后进行了 PCR。40 份样本的 95% 机率值分别为：离心法 27.25 IU/ml，真空法 9.30 IU/ml。

Specifications

Features	Specifications
Applications	PCR、印迹
Yield	每个拭子 1–2 µg
Format	96 孔板
For automated processing	BioRobot Universal System、BioRobot Genotyping、BioRobot 9604
Sample amount	每孔 1 个拭子
Processing	自动
Elution volume	150 µl
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	基因组 DNA
Main sample type	拭子
Technology	硅膜技术
Time per run or per prep	<2 小时

资源

For automated purification of genomic DNA from buccal swabs using the BioRobot Universal System, BioRobot Genotyping, or BioRobot 9604

Download Safety Data Sheets for QIAGEN product components.

For automated purification of genomic DNA from buccal swabs using the BioRobot Universal System, BioRobot Genotyping, or BioRobot 9604

Publications

Estrogen sulfation genes, hormone replacement therapy, and endometrial cancer risk.

Rebbeck TR; Troxel AB; Wang Y; Walker AH; Panossian S; Gallagher S; Shatalova EG; Blanchard R; Bunin G; DeMichele A; Rubin SC; Baumgarten M; Berlin M; Schinnar R; Berlin JA; Strom BL;

J Natl Cancer Inst; 2006; 98 (18):1311-20 2006 Sep 20 PMID:16985250

Isolation of genomic DNA from buccal swabs for forensic analysis, using fully automated silica-membrane purification technology.

Hanselle T; Otte M; Schnibbe T; Smythe E; Krieg-Schneider F;

Leg Med (Tokyo); 2003; 5 Suppl 1 :S145-9 2003 Mar PMID:12935575

FAQ

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

FAQ ID -761

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12