QIAamp 96 DNA QIAcube HT Kit

用于从血液、细胞和组织中自动完成高通量的总 DNA 分离

S_3616_LS_QIAamp96DNA_s

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QIAamp 96 DNA QIAcube HT Kit (5)

Cat. No. / ID:  51331

用于 480 次制备:QIAamp 96 孔板、QIAGEN Proteinase K、缓冲液
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KitAccessories
QIAamp 96 DNA QIAcube HT Kit
QIAcube HT Plasticware
QIAamp 96 DNA QIAcube HT Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 简单可靠的自动处理,节省成本和时间
  • 适用于各种样本类型的标准化方法
  • 即使是专门样本也能获得高产量
  • 在下游分析中表现出色的高品质 DNA
  • 适用于一系列起始材料的优化方案

Product Details


QIAamp 96 DNA QIAcube HT Kit 可通过简单、自动化的方式从血液、细胞和组织样本中纯化总 DNA(例如基因组、线粒体和病原体)。使用成熟可靠的 QIAamp 硅胶膜技术,以方便的 96 孔格式去除污染物和抑制物,从而获得可随时供下游分析使用的高品质核酸。该试剂盒设计用于 QIAcube HT System。

Performance

QIAamp 96 DNA QIAcube HT Kit 可以 96 孔格式快速可靠地自动纯化总 DNA。结果的可重现性高,孔间 DNA 回收率没有明显差异(见图 可重现的 96 孔 DNA 纯化)。
使用 QIAamp 96 DNA QIAcube HT Kit 可快速、简易、可靠地从血样中自动纯化总 DNA。可以处理含有常见抗凝剂的新鲜或冷冻全血,得到的 DNA 与其他 QIAGEN 纯化解决方案得到的 DNA 具有相同的高品质(见图 纯化基因组 DNA 的琼脂糖凝胶 人血基因组 DNA 分析)。
QIAcube HT 和专用的 QIAamp 96 DNA QIAcube HT Kit 可让用户提高样本纯化通量而又不必牺牲质量或可靠性。与其他 QIAGEN DNA 纯化解决方案类似,该操作流程提供的高产量纯 DNA 在下游检测中表现出色(见图 下游检测中的高性能 从组织中纯化基因组 DNA)。
See figures

Principle

QIAamp 96 DNA QIAcube HT Kit 可从包括新鲜或冷冻组织、细胞和血液在内的各种样本来源自动纯化总 DNA(例如基因组、线粒体和病原体)。适合使用以普通抗凝剂(如柠檬酸盐、肝素或 EDTA)处理的样本。该操作流程产生的高品质 DNA 在 PCR 和其他酶反应中表现出色。
QIAamp 96 DNA QIAcube HT Hit 将硅胶膜的选择性结合特性与高通量 96 孔格式相结合,专为在 QIAcube HT 仪器上全自动同时处理 24–96 个样本而设计。

 

试剂盒规格
规格 说明
样本数量 24–96 个样本(以 8 为增量进行处理)
样本输入体积 血液:最多 200 μl(样本量小于 200 μl 时,加入 PBS)
细胞:最多 5 x 106 个细胞
组织:最多 20 mg 组织(脾脏、肝脏或肺组织为 10 mg)
洗脱体积 200 μl
持续时间 96 个样本约 96 分钟
24 个样本约 45 分钟

Procedure

使用 QIAcube HT 仪器可以快速简单地完成 QIAamp 96 DNA QIAcube HT 操作流程。首先使用 QIAGEN Proteinase K 对样本进行裂解。调整缓冲条件以提供最佳的 DNA 结合条件,然后将裂解物装载到 QIAamp 96 板上。抽真空时,DNA 会选择性地与 QIAamp 硅胶膜结合,污染物则会通过。通过三个高效的洗涤步骤去除残留的污染物和酶抑制物。真空条件下,纯 DNA 在 200 µl Buffer AE 中一步完成洗脱。

Applications

QIAamp 96 DNA QIAcube HT Kit 提供的高品质 DNA 可随时用于各种下游检测,包括以下应用领域:
生命科学研究
牲畜饲养
血统基因分型
兽医病原体研究
常规执行的测试

Supporting data and figures

Resources

实验方案软件 (2)
Q Protocol notes for purification of genomic DNA from blood, cells, and soft tissue on the QIAcube HT using the QIAamp 96 DNA QIAcube HT Kit; use with the QIAamp 96 DNA QIAcube HT V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
试剂盒操作手册 (2)
Handbook for purification of genomic DNA from blood, cells, and soft tissue on the QIAcube HT using the QIAamp 96 DNA QIAcube HT Kit. For use with QIAcube HT Operating Software 4.17; not compatible with QIAcube HT Prep Manager Software.
For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
快速启动实验方案 (3)
For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
产品介绍与指南 (1)
用户使用手册 (3)
For use with the QIAcube HT Prep Manager Software
For QIAcube HT and QIAxtractor
For QIAcube HT and QIAxtractor
Safety Data Sheets (1)
Certificates of Analysis (1)

FAQ

Can I run the QIAcube HT with the QIAxtractor software or vice versa?

No, the two software applications are not interchangeable.

 

Additionally, the QIAcube HT and QIAxtractor software cannot be installed on the same computer. The QIAcube HT software versions is v4.17 or higher, while the QIAxtractor software version is v4.16 or lower.

FAQ ID -9102
For what batch size is the QIAcube HT Plasticware designed?
The QIAcube HT Plasticware content is calculated for use with the extraction protocol working in batches of 96 samples. If running smaller batches and/ or the transfer protocol, additional consumables are required. 
FAQ ID - 4010
Can I upgrade a Corbett CAS-1820 to a QIAcube HT?

No, this upgrade is not possible.

FAQ ID -9104
What file is required for reporting a QIAcube HT or QIAxtractor issue?

The Support Packages are important when reporting an issue to QIAGEN Technical Services. Upon completion of each run, the software automatically saves a support package.

 

To retrieve a support package, click Help in the QIAcube HT or QIAxtractor software and select Choose support package. In the new windows, select the support package that corresponds to the issue and send it to QIAGEN Technical Services.

 

FAQ ID -9100
I am getting vacuum errors on my QIAcube HT. What should I do?

1. Check the vacuum tubing to make sure it is not pinched or obstructed in some way. Sometimes the tubing can become pinched against the bench top when the vacuum station is placed too far from the instrument.

Waste bottle tubing

2. Make sure that the channeling adapter is properly placed. Check if the carriage is moving the plate completely from the waste chamber position to the elution chamber position. If the calibration seems incorrect, the instrument may need to be re-taught at this position.

Adaptors

3. When using less than a full plate, make sure all the unused columns are covered with an adhesive seal.

Also, make sure each column of 8 samples is full. When processing a number of samples that results in < 8 samples per column, use water as a substitute in the empty positions until the column is filled. This ensures even vacuum across all unsealed well positions.

4. Make sure correct plates are used for the selected protocol.

5. When the vacuum is on, please listen for any hissing noise from the tubing that is coming down to the waste container. This could indicate leaks in the tubing.

If the issue persists, please contact QIAGEN Technical Service.



FAQ ID -9106
What do I do if the QIAcube HT or QIAxtractor software runs in virtual mode while the instrument is connected to the computer?

Make sure the QIAcube HT or QIAxtractor software uses the same COM port number as assigned to the QIAcube HT or QIAxtractor instrument on the computer. The COM port setting can be checked by clicking Options\Robot Setup\Select COM port in the QIAcube HT or QIAxtractor software.

 

Ensure that the cable between the instrument and the computer is securely inserted. Power on the instrument first, and then launch the QIAxtractor/QIAcube HT software. 

 

The instrument has both RS-232 (serial) and USB interfaces for computer connection. Try an RS-232 cable if the communication cannot be established between the instrument and computer with a USB cable.

 

If the issue persists, please contact QIAGEN Technical Services.

 

FAQ ID -9101