Real-Time PCR Kits | qPCR Enzymes & Kits

Choose the right kit for your application

Real-time PCR (qPCR) enables sensitive and quantitative detection of DNA and RNA targets.

Different assay chemistries and workflows are optimized for specific experimental needs, including gene expression analysis, pathogen detection and multiplex assays.

Use the overview below to identify the qPCR solution that best fits your template type, detection chemistry and application.

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Product category	Your application goal	Recommended product family	Product features	When to choose
One-step RT-qPCR master mixes qPCR master mixes	High-sensitivity and fast real-time PCR and RT-qPCR DNA/cDNA or RNA quantification (e.g., gene expression analysis)	QuantiNova qPCR Kits	SYBR Green Probe based Multiplex One-step RT-qPCR Guard-protected antibody hot start PCR Visual pipetting and internal control RNA	When maximum sensitivity, fast cycling or improved specificity is required with inbuilt safety and convenience features
One-step RT-qPCR master mixes qPCR master mixes	Routine real-time PCR and RT-PCR with established workflows DNA/cDNA or RNA quantification (e.g., gene expression analysis)	QuantiTect qPCR Kits	SYBR Green Probe based Multiplex One-step RT-qPCR Chemically-mediated hot start PCR	When robust and well-established qPCR performance is required for standard applications
Reverse transcription / cDNA synthesis kits	cDNA generation for downstream qPCR applications	QuantiNova Reverse Transcription Kits QuantiTect Reverse Transcription Kits Omniscript	Reverse transcription Integrated gDNA removal Optimized primer mix provided	When cDNA synthesis, archiving or multiple downstream assays are required
Pathogen detection	Pathogen and virus detection	QuantiNova Pathogen QuantiFast Pathogen QIAprep&amp	Probe based Multiplex Direct qPCR from crude sample (QIAprep&amp)	When analyzing pathogens: viruses, bacteria or parasites (e.g., Plasmodium spp.)
microRNA analysis	Sensitive and specific miRNA quantification	miRCURY LNA miRNA PCR Starter Kit miRCURY Probe PCR Kits miRCURY LNA SYBR Green PCR Kits	SYBR Green Probe based Guard-protected antibody hot start PCR	When analyzing mature miRNA targets with high specificity
Specialized qPCR applications	Perform qPCR workflows optimized for specific targets, sample types or applications	Ultra-Clean Production Kits Certal Residual DNA Detection Kits mericon Pathogen Detection Assay mericon Ingredient Authentication Assay	Probe based One-step from sample	When analyzing residual host-cell DNA or specific applications like food pathogens
Direct qPCR from cells	Direct RT-qPCR from cultured cells without RNA purification	FastLane Cell RT-PCR Kits FastLane Cell cDNA Kit	Direct qPCR SYBR Green Probe based Multiplex One-step RT-qPCR Chemically-mediated hot start PCR	When minimizing sample preparation and processing time

One-step RT-qPCR

	Detection format	Total RNA input range	Amplicon length	Multiplex compatibility	RT hot start	UNG treatment possible	gDNA removal	Internal control RNA	Room-temperature setup	Visual pipetting
QuantiNova Probe RT-PCR Kits	Probe based	≤400 ng – 100 fg	60–300 bp	2-plex	Yes	No	Yes	Yes	Yes	Blue and yellow
QuantiNova Multiplex RT-PCR Kits	Probe based	≤800 ng	60–300 bp	5-plex	Yes	No	No	Yes	Yes	Blue and yellow
QuantiNova Pathogen Kits	Probe based	N/A	60–300 bp	4-plex	Yes	No	No	Yes	Yes	Blue and yellow
QuantiNova SYBR RT-PCR Kits	SYBR Green	100 fg – 200 ng	60–150 bp	N/A	Yes	No	No	Yes	Yes	Blue and yellow
QuantiTect Probe RT-PCR Kits	Probe based	1 pg – 500 ng	60–300 bp	Single-plex	No	Possible	No	No	No	No
QuantiTect Multiplex RT-PCR Kits	Probe based	≤250 ng	60–300 bp	4-plex	No	Possible	No	No	No	No
QuantiTect SYBR RT-PCR Kits	SYBR Green	≤500 ng	60–300 bp	N/A	No	Possible	No	No	No	No
QuantiTect Virus Kits	Probe based	N/A	60–300 bp	4-plex	No	No	No	No	No	No
QuantiFast Pathogen RT-PCR Kits	Probe based	N/A	60–300 bp	4-plex	No	No	No	Yes	No	No

qPCR master mixes (two-step)

	Detection format	Total input range	Amplicon length	Sample type	Multiplex compatibility	Hot start	UNG treatment possible	Fast cycling	Reaction volume (µL)	Room-temperature setup	Visual pipetting
QuantiNova Probe PCR Kits	Probe based	≤100 ng	60–200 bp	gDNA, cDNA	2-plex	Antibody	No	Yes	20	Yes	Blue and yellow
QuantiNova Multiplex PCR Kits	Probe based	≤800 ng	60–200 bp	gDNA, cDNA	5-plex	Antibody	No	Yes	20	Yes	Blue and yellow
QuantiNova SYBR PCR Kits	SYBR Green	≤100 ng	60–200 bp	gDNA, cDNA	N/A	Antibody	No	Yes	20	Yes	Blue and yellow
QuantiNova Pathogen + IC Kits	Probe based	N/A	60–300 bp	gDNA, cDNA	4-plex	Antibody	No	Yes	20	Yes	Blue and yellow
QuantiTect Probe Kits	Probe based	<1,000 ng	60–300 bp	gDNA, cDNA	Single-plex	Chemical	Possible	No	50	Yes	No
QuantiTect Multiplex Probe Kits	Probe based	<500 ng	60–200 bp	gDNA, cDNA	4-plex	Chemical	Possible	No	50	Yes	No
QuantiTect Probe UNG Kits	Probe based	<1,000 ng	60–300 bp	gDNA, cDNA	Single-plex	Chemical	UNG included	No	50	Yes	No
QuantiTect SYBR Kits	SYBR Green	<500 ng	100–500 bp	gDNA, cDNA	N/A	Chemical	Possible	No	50	Yes	No
QuantiTect SYBR Green PCR +UNG Kits	SYBR Green	<500 ng	100–500 bp	gDNA, cDNA	N/A	Chemical	UNG included	No	50	Yes	No

Reverse transcription / cDNA synthesis kits

	gDNA removal	Total RNA input range	Optimal reaction time	Oligo(dt)	Random	Gene specific
QuantiNova Reverse Transcription Kit	Yes	10 pg – 5 µg	10 min (RT step)	Yes	Yes	Yes
QuantiTect Reverse Transcription Kit	Yes	10 pg – 1 µg	15 min (RT step)	Yes	Yes	Yes
miRCURY LNA RT Kit	No	5 ng/µL	60 min (RT step)	poly-A and universal Tag added	N/A	miRCURY LNA assays

Pathogen detection

	1-step RT-qPCR	Detection format	Sample type	Amplicon length	Multiplex PCR	RT hot start	UNG treatment	gDNA removal	Visual pipetting	Room-temperature setup	Internal control RNA
QuantiNova Pathogen Kit	Yes	Probe based	Isolated DNA/RNA	60–300 bp	4-plex	Yes	No	No	Blue and yellow	Yes	Yes
QuantiFast Pathogen RT-PCR Kit	Yes	Probe based	Isolated DNA/RNA	60–150 bp (more possible)	4-plex	No	No	No	No	No	Yes
QIAprep&amp Viral RNA UM Kit	Yes	Probe based	Buccal swabs, gargle, saliva	60–300 bp	4-plex	Yes	No	No	Blue and yellow	Yes	Yes
QIAprep&amp Plasmodium Kit	Yes	Probe based	Liquid blood, dried blood spots (DBS)	60–300 bp	4-plex	No	No	No	Blue and yellow	Yes	Part of Plasmodium assay kit

microRNA analysis

	Purpose	Detection format	Reaction volume (µL)	Sample type	Fast cycling	Multiplex compatibility	UNG treatment	Amplicon length	Hot start	Input	Room-temperature setup	Visual pipetting
miRCURY Probe PCR Kits	miRNA detection, quantification and profiling	Probe based	10 µL or 20 µL	miRNA	Yes	4-plex	No	<40 bp	Antibody	10 pg – 200 ng	Yes	Blue master mix
miRCURY LNA SYBR Green PCR Kits	miRNA detection, quantification and profiling	SYBR Green	10 µL or 20 µL	miRNA	Yes	N/A	No	<40 bp	Antibody	10 pg – 200 ng	Yes	Blue master mix

Specialized qPCR applications

	Purpose	Detection format	Reaction volume (µL)	Sample type	Fast cycling	Multiplex compatibility	UNG treatment	Amplicon length	Hot start	Input	Room-temperature setup	Visual pipetting
UCP PCR Kits	Ultra-clean production. Nucleic acid-depleted, ultra-clean reagents suited for processes that are prone to contaminating background DNA.	SYBR Green	20	gDNA, cDNA	Yes	N/A	No	60–300 bp	Antibody	10 fg – 100 ng	Yes	Blue and yellow
UCP Probe PCR Kit	Ultra-clean production. Nucleic acid-depleted, ultra-clean reagents suited for processes that are prone to contaminating background DNA.	Probe based	20	gDNA, cDNA	Yes	2-plex	No	60–300 bp	Antibody	≤100 ng	Yes	Blue and yellow
Type-It Fast SNP Probe kit	Highly accurate SNP genotyping	Probe based	25	gDNA	Yes	2-plex	No	60–300 bp	Chemical	0.1–100 ng	Yes	No
Certal Residual DNA Detection Kits	Detection of residual CHO host cell gDNA	Probe based	25	gDNA	No	2-plex	UNG included	60–200 bp	Chemical	>3 fg	Yes	No
mericon Ingredient Authentication Assay	Authentication of food and animal feed ingredients	Probe based	20	gDNA	Yes	Single-plex	No	60–200 bp	Chemical	0.1–50 ng	Yes	No
mericon Pathogen Detection Assay	Detection of pathogen DNA in food and animal feed	Probe based	20	gDNA	Yes	Single-plex	No	60–200 bp	Chemical	10–2,500 copies of bacterial DNA	Yes	No

Real-Time PCR Enzymes & Kits