Do I have to use a ramp time of 2°C/sec on the LightCycler when using Quantitect Primer Assays?

We strictly recommend to use a ramp time of 2°C/sec when running QuantiTect Primer Assays on the LightCycler because cycling is much more robust when compared to using 20°C/sec for ramping. If optimal assay conditions have been established using our recommended ramp time, customers can of course try to use 20°C/sec ramping for denaturation and annealing steps at their own risk.


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