Can the miScript II RT Kit be used to quantify piRNAs?

No, the miScript II RT Kit cannot be used to quantify piRNAs. Small RNAs possessing a 2’-O-methyl (2’-O-Me) modification on their 3’ terminal base, such as plant mature miRNAs and piwi-interacting RNAs (piRNAs), are refractory to polyadenylation. As a result, they cannot be efficiently reverse transcribed using a polyadenylation-based cDNA synthesis approach, which is the underlying mechanism of various reverse transcription kits including the miScript II RT Kit.


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