What is the smallest amount of DNA I can put into a 50 ul REPLI-g reaction?
The minimum amount of DNA for successful WGA with the REPLI-g Kit is 10 ng. Optimal amplification is achieved with 100 ng or more of genomic DNA template. Comprehensive and accurate amplification of the human genome can be accomplished directly from 300 or more human cells, from tissue culture, or from 1.0 ul whole blood without prior DNA purification. Amplification is dependent upon the quality of input DNA.