What does transcript variant mean when searching for specific QuantiTect Primer Assays?

Many genes have more than one transcript due to alternative splicing. We refer to the various transcripts (splice variants) for a particular gene as transcript variants. When searching GeneGlobe for a QuantiTect Primer Assay, you may locate a link below the assay name saying 'Show Primer Assays for transcript variants', whenever there are assays for transcript variants available. If there is no such link below an assay name, then the specific Primer Assay can detect all transript variants simultaneously.



Human adrenergic, alpha-1A-, receptor ADRA1A

For this specific QuantiTect Primer Assay there are assays for transcript variants available which can be accessed via the link below the QuantiTect Primer Assay name.

Assays for transcript variants are named as follows:

Hs_ADRA1A_vb.1_SG QuantiTect Primer Assay (200)

Hs:            human

ADRA1A:   adrenergic, alpha-1A-, receptor

vb.1:         transcript variant b for a specific RefSeq ID Number; assay version 1

SG:           assay is for SYBR Green based real-time RT-PCR

(200):        200 x 50 µl reactions

In this specific example, the letters 'vb.1' in 'Hs_ADRA1A_vb.1_SG' are used to indicate that the assay is specific for the transcript variant with RefSeq ID NM_033302, and that the Primer Assay is the first version for this particular transcript variant.

Click the 'View details' link for a particular assay to find out which transcript variant it is detecting.


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