Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA?

Yields of amplified DNA using the REPLI-g UltraFast Mini Kit can vary depending on DNA quality. With any whole genome amplification reaction, DNA fragmentation and the presence of inhibitors can reduce the amount of DNA obtained. DNA fragmentation can be caused by many factors including heating of the DNA, shearing of the DNA, multiple freeze-thaw cycles, and sample storage and handling conditions.



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