At which step in the REPLI-g FFPE protocol should PicoGreen quantification be done?

When using PicoGreen for quantification of REPLI-g FFPE amplifiied DNA, perform the assay after step 15 of the REPLI-g FFPE protocol, prior to the heating step at 95°C.

PicoGreen detects double-stranded DNA with high sensitivity. If the DNA was quantified after denaturation at 95°C using PicoGreen, multiply the yield by a factor of 2 to compensate for the use of single-stranded DNA.



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