What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit?

An ideal amplicon size for bisulfite converted DNA (e.g., using EpiTect Bisulfite Kits) is up to 250 bp. However, we amplified fragments up to 700 bp successfully using bisulfite converted DNA as a template for the EpiTect MSP Kit. Note that the time for the elongation step needs to be increased for such long amplicons.



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