Should total RNA or small enriched RNA be used as the starting material for the QIAseq miRNA Library Kit?
Total RNA containing miRNA is the required starting material for the QIAseq miRNA Library Kit. It is not necessary to enrich for small RNA. If, however, it is desired to work with small RNA samples, small RNA represents approximately 10% of total RNA amounts. Therefore, as an example, 100 ng of total RNA translates to 10 ng small RNA; as a result, you would use the adapter and RT primer dilutions recommended for 100 ng total RNA (when working with 10 ng small RNA).