Can I use any type of DNA extraction method to purify template DNA for amplification using the REPLI-g Mitochondrial DNA Kit?
Intact mitochondrial DNA is necessary for successful amplification with the REPLI-g Mitochondrial DNA Kit. Most DNA extraction methods are optimized for the isolation of linear nuclear DNA rather than for mitochondrial DNA. Furthermore, any harsh conditions leading to fragmentation of double stranded DNA must be avoided. We recommend the use of QIAamp DNA Kits, which are available in various formats and optimized for different sample materials.