3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)?

After reconstitution of the lyophilized primers in 1.1ml of TE, pH 8.0, the QuantiTect Primer Assay will be at 10X stock concentration.  It should be used at 1X final concentration in the PCR/RT-PCR reaction (eg. for a 25ul reaction, 1/10th of the total volume, or 2.5ul, should be primer).  The exact concentration of the primers is proprietary.


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